WB (Western Blot)
(Detection of human PPM1B by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, A-549, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPM1B antibody AAA211199 (lot AAA211199-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human PPM1B Polyclonal Antibody | anti-PPM1B antibody
Rabbit anti-PPM1B Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
PPM1B, Antibody; Rabbit anti-PPM1B Antibody, Affinity Purified; PP2CBETA; protein phosphatase 2C-like protein; protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform; Protein phosphatase 2C isoform beta; Ser/Thr protein phosphatase type 2C beta 2 isoform; PPC2BETAX; PP2C-beta; PP2CB; PP2C-beta-X; protein phosphatase 1B; Protein phosphatase 1B; protein phosphatase, Mg2+/Mn2+ dependent 1B; anti-PPM1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-PPM1B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 429 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PPM1B by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, A-549, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPM1B antibody AAA211199 (lot AAA211199-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human PPM1B by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, A-549, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPM1B antibody AAA211199 (lot AAA211199-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human PPM1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPM1B antibody AAA211199 (lot AAA211199-2) used for IP at 6 ug per reaction. PPM1B was also immunoprecipitated by a previous lot of this antibody (lot AAA211199-1). For blotting immunoprecipitated PPM1B, AAA211199 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human PPM1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPM1B antibody AAA211199 (lot AAA211199-2) used for IP at 6 ug per reaction. PPM1B was also immunoprecipitated by a previous lot of this antibody (lot AAA211199-1). For blotting immunoprecipitated PPM1B, AAA211199 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-PPM1B antibody
Members of the protein phosphatase 2C (PP2C) family of isoenzymes are serine/threonine protein phosphatases that act as monomers, are magnesium or manganese dependent, and are structurally distinct from PP1, PP2A, and PP2B protein phosphatases. There are 14 mammalian PP2C genes that encode for members of the PP2C family of isoenzymes. Members of this family include PP2C alpha (PPM1A), PP2C beta (PPM1B), PP2C gamma (PPM1G), and PP2C delta (PPM1D). PPM1B represents the protein phosphatase 2C (PP2C) beta isoform 1. Six splice variants of the PPM1B have been identified PPM1B activation by lipophilic compounds has been demonstrated to be associated with the induction of apoptosis PPM1B activity has been shown to be involved in cell cycle control and the dephosphorylation of cyclin dependent kinases cdk2 and cdk6.
Product Categories/Family for anti-PPM1B antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The PPM1B ppm1b (Catalog #AAA211199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PPM1B Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1B ppm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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