IF (Immunofluorescence)
(AAA328988 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328988 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit PP2C alpha Polyclonal Antibody | anti-PPM1B antibody
PP2C alpha Antibody
Gene Names
PPM1B; PP2CB; PP2CBETA; PP2C-beta; PPC2BETAX; PP2C-beta-X
Reactivity
HumanPredicted Reactivity: Pig(100%), Bovine(100%), Horse(86%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PP2C alpha, Antibody; PP2C alpha Antibody; MGC21657; OTTHUMP00000158953; OTTHUMP00000158954; OTTHUMP00000158955; OTTHUMP00000202394; OTTHUMP00000202395; OTTHUMP00000202399; PP2C beta; PP2C beta X; PP2C-beta; PP2CB; PP2Cbeta; PPC2BETAX; PPM 1B; Ppm1b; PPM1B_HUMAN; Protein phosphatase 1B (formerly 2C) magnesium dependent beta isoform; Protein phosphatase 1B; Protein phosphatase 1B magnesium dependent beta isoform; Protein phosphatase 2C beta isoform; Protein phosphatase 2C isoform beta; Protein phosphatase 2C like protein; Protein phosphatase; Mg2+/Mn2+ dependent; 1B; anti-PPM1B antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(86%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(86%), Rabbit(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
PP2C alpha Antibody detects endogenous levels of total PP2C alpha.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
192
Applicable Applications for anti-PPM1B antibody
ELISA
Immunogen
A synthesized peptide derived from human PP2C alpha, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328988 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328988 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328988 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328988 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells , using PP2C alpha Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells , using PP2C alpha Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PPM1B antibody
Function: Enzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
Post Translational Modifications: Isgylation negatively regulates its activity.N-myristoylation is essential for the recognition of its substrates for dephosphorylation.
Subcellular Location: Cytoplasm>Cytosol. Membrane>Lipid-anchor. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.
Tissue Specificity: Highly expressed in heart and skeletal muscle.
Subunit Structure: Monomer (By similarity). Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.
Similarity: Belongs to the PP2C family.
Post Translational Modifications: Isgylation negatively regulates its activity.N-myristoylation is essential for the recognition of its substrates for dephosphorylation.
Subcellular Location: Cytoplasm>Cytosol. Membrane>Lipid-anchor. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.
Tissue Specificity: Highly expressed in heart and skeletal muscle.
Subunit Structure: Monomer (By similarity). Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.
Similarity: Belongs to the PP2C family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)52kD.
Predicted Molecular Weight: (Calculated)53kDa.
Predicted Molecular Weight: (Calculated)53kDa.
NCBI Official Full Name
protein phosphatase 1B isoform 3
NCBI Official Synonym Full Names
protein phosphatase, Mg2+/Mn2+ dependent 1B
NCBI Official Symbol
PPM1B
NCBI Official Synonym Symbols
PP2CB; PP2CBETA; PP2C-beta; PPC2BETAX; PP2C-beta-X
NCBI Protein Information
protein phosphatase 1B
UniProt Protein Name
Protein phosphatase 1B
UniProt Gene Name
PPM1B
UniProt Synonym Gene Names
PP2CB; PP2C-beta
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Product Notes
The PPM1B ppm1b (Catalog #AAA328988) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP2C alpha Antibody reacts with Human Predicted Reactivity: Pig(100%), Bovine(100%), Horse(86%), Rabbit(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's PP2C alpha can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the PPM1B ppm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2C alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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