IHC (Immunohiostchemistry)
(Immunochemical staining of human PPM1B in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human PPM1B Polyclonal Antibody | anti-PPM1B antibody
Anti-PPM1B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPM1B, Antibody; Anti-PPM1B Antibody, Rabbit Polyclonal; PPM1B Antibody, Rabbit PAb, Antigen Affinity Purified; protein phosphatase, Mg2+/Mn2+ dependent, 1B; Anti-PP2C-beta Antibody; Anti-PP2C-beta-X Antibody; Anti-PP2CB Antibody; Anti-PP2CBETA Antibody; Anti-PPC2BETAX Antibody; anti-PPM1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPM1B
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PPM1B antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human PPM1B fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PPM1B fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human PPM1B in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human PPM1B in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-PPM1B rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:57 kDa)
WB (Western Blot)
(Anti-PPM1B rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:57 kDa)
Related Product Information for anti-PPM1B antibody
PPM1B is a metal-dependent serine/threonine protein phosphatase, with a similar structure and function to the well-known oncogene in breast cancer, PPM1D (WIP1). PPM1B is a member of the protein phosphatase 2C family and plays a role in negatively regulating p53 and NF-kappaB thereby possibly attenuating the gene expression program of cellular senescence. PPM1B is a critical regulator of both p38 MAPK-dependent and independent senescence pathways during the normal cellular aging process and catalyzes the dephosphorylation of a variety of phosphorylated proteins. PPM1B plays a negative role in the activation of the p38-RB1-E2F1 pathway and that targeting PPM1B could be useful in certain types of cancer by stimulating chemotherapy-induced cell death. Protein phosphatase PPM1B is reported to dephosphorylate IKKbeta, thereby terminating IKKbeta-mediated NF-kappaB activation.
Product Categories/Family for anti-PPM1B antibody
References
Yang J, et al. (2016) Mir-186 downregulates protein phosphatase ppm1b in bladder cancer and mediates g1-s phase transition. Tumour Biol 37 (4): 4331-4341.Park JH, et al. (2014) Ppm1b depletion induces premature senescence in human imr-90 fibroblasts. Mech Ageing Dev 138 45-52.Miller RE, et al. (2018) Ppm1b depletion in u2os cells supresses cell growth through rb1-e2f1 pathway and stimulates bleomycin-induced cell death. Biochem Biophys Res Commun 500 (2): 391-397.Ishii N, et al. (2019) A heterozygous deficiency in protein phosphatase ppm1b results in an altered ovulation number in mice. Mol Med Rep 19 (6): 5353-5360.
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Product Notes
The PPM1B (Catalog #AAA256034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPM1B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPM1B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PPM1B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPM1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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