WB (Western Blot)
(Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-PPP1CA-T320 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
Rabbit anti-Human, Mouse PPP1CA-T320 Polyclonal Antibody | anti-PPP1CA-T320 antibody
Phospho-PPP1CA-T320 pAb
Gene Names
PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
PPP1CA-T320, Antibody; Phospho-PPP1CA-T320 pAb; PP-1A; PP1A; PP1alpha; PPP1A; anti-PPP1CA-T320 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Sequence Length
341
Applicable Applications for anti-PPP1CA-T320 antibody
WB (Western Blot)
Immunogen
A phospho specific peptide corresponding to residues surrounding T320 of human PPP1CA
Immunogen Species
Human
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-PPP1CA-T320 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-PPP1CA-T320 antibody at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-PPP1CA-T320 antibody
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-PPP1CA-T320 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 32kDa; 37kDa; 38kDa
Observed: 38kDa
Observed: 38kDa
NCBI Official Full Name
serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 3
NCBI Official Synonym Full Names
protein phosphatase 1 catalytic subunit alpha
NCBI Official Symbol
PPP1CA
NCBI Official Synonym Symbols
PP1A; PP-1A; PPP1A; PP1alpha
NCBI Protein Information
serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Gene Name
PPP1CA
UniProt Synonym Gene Names
PPP1A; PP-1A
Similar Products
Product Notes
The PPP1CA-T320 ppp1ca (Catalog #AAA281285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PPP1CA-T320 pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1CA-T320 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PPP1CA-T320 ppp1ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP1CA-T320, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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