Standard Curve (Sample)
Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit | PPP1CA elisa kit
Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit
Gene Names
PPP1CA; PP1A; PP-1A; PPP1A; PP1alpha
Reactivity
Rat
Synonyms
Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit; N/A; Rat Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit ELISA Kit; PPP1CA elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of sST2. No significant cross-reactivity or interference between sST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sST2 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
330
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PPP1CA elisa kit
Intended Uses: This sST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sST2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: sST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sST2 antibody and an sST2-HRP conjugate. The assay sample and buffer are incubated together with sST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sST2 concentration since sST2 from samples and sST2-HRP conjugate compete for the anti-sST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sST2 from the sample, fewer sites are left to bind sST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sST2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: sST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sST2 antibody and an sST2-HRP conjugate. The assay sample and buffer are incubated together with sST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sST2 concentration since sST2 from samples and sST2-HRP conjugate compete for the anti-sST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sST2 from the sample, fewer sites are left to bind sST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sST2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,631 Da
NCBI Official Full Name
serine/threonine-protein phosphatase PP1-alpha catalytic subunit isoform 1
NCBI Official Synonym Full Names
protein phosphatase 1 catalytic subunit alpha
NCBI Official Symbol
PPP1CA
NCBI Official Synonym Symbols
PP1A; PP-1A; PPP1A; PP1alpha
NCBI Protein Information
serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Protein Name
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
UniProt Gene Name
PPP1CA
UniProt Synonym Gene Names
PPP1A; PP-1A
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Product Notes
The Rat PPP1CA ppp1ca (Catalog #AAA247315) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247315 ELISA Kit recognizes Rat PPP1CA. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Phosphatase PP1-alpha Catalytic Subunit, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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