Rabbit PPP1CC Polyclonal Antibody | anti-PPP1CC antibody

Anti-PPP1CC Antibody, Rabbit Polyclonal

Cat. Num. AAA255107

• Gene Names: PPP1CC; PP1C; PP-1G; PPP1G
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, Xenopus tropicalis (Species predicted to react based on 100% sequence homology)
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PPP1CC, Antibody; Anti-PPP1CC Antibody, Rabbit Polyclonal; PPP1CC Antibody, Rabbit PAb, Antigen Affinity Purified; protein phosphatase 1, catalytic subunit, gamma isozyme; Anti-PP-1G Antibody; Anti-PP1C Antibody; Anti-PPP1G Antibody; anti-PPP1CC antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovine, Xenopus tropicalis (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PPP1CC
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PPP1CC antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human PPP1CC
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PPP1CC, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence staining of PPP1CC in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PPP1CC polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)

WB (Western Blot)

(Anti-PPP1CC rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:37 kDa)

Related Product Information for anti-PPP1CC antibody

Protein phosphatase 1, catalytic subunit, gamma isozyme (PPP1CC) participates in cell proliferation, differentiation, and apoptosis. Using fluorescent in situ hybridization (FISH) method, a gene encoding the catalytic subunit of protein phosphatase type 1 gamma (PPP1CC) was mapped to human 12q24.1-q24.2, rat 7 q22, and mouse 10C. These results indicate that the PPP1CC is a member of the conserved synteny group between rat chromosome 7, mouse chromosome 10, and human chromosome 12. The Ppp1cc gene encodes two alternatively spliced variants, PP1 gamma1 (PPP1CC1) and PP1 gamma2 (PPP1CC2). Global deletion of the Ppp1cc gene, which causes loss of both isoforms, results in male infertility due to impaired spermatogenesis. This phenotype was assumed to be due to the loss of PPP1CC2, which is abundant in testis. While PPP1CC2 is predominant, other PP1 isoforms are also expressed in testis.

References

Sinha N, et al. (2013) Selective ablation of ppp1cc gene in testicular germ cells causes oligo-teratozoospermia and infertility in mice. Biol Reprod 89 (5): 128.Saadat M, et al. (1996) Assignment of the gene encoding type 1 gamma protein phosphatase catalytic subunit (ppp1cc) on human, rat, and mouse chromosomes. Jpn J Hum Genet 41 (1): 159-165.MacLeod G, et al. (2014) Ppp1cc2 can form a kinase/phosphatase complex with the testis-specific proteins tssk1 and tsks in the mouse testis. Reproduction 147 (1): 1-12.Liu X, et al. (2017) Ppp1cc is associated with astrocyte and microglia proliferation after traumatic spinal cord injury in rats. Pathol Res Pract 213 (11): 1355-1364.

NCBI and Uniprot Product Information

• NCBI GI #: 548573
• NCBI GeneID: 5501
• UniProt Accession #: P36873
• Molecular Weight: 323
• NCBI Official Full Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• NCBI Official Synonym Full Names: protein phosphatase 1, catalytic subunit, gamma isozyme
• NCBI Official Symbol: PPP1CC
• NCBI Official Synonym Symbols: PP1C; PP-1G; PPP1G
• NCBI Protein Information: serine/threonine-protein phosphatase PP1-gamma catalytic subunit; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase PP1-gamma catalytic subunit
• UniProt Gene Name: PPP1CC
• UniProt Synonym Gene Names: PP-1G
• UniProt Entry Name: PP1G_HUMAN