IF (Immunofluorescence)
(Figure 2. IF analysis of PRDM4 using anti-PRDM4 antibody (AAA127737).PRDM4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PRDM4 Antibody (AAA127737) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Rabbit PRDM4 Polyclonal Antibody | anti-PRDM4 antibody
Anti-PRDM4 Antibody Picoband
Gene Names
PRDM4; PFM1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
PRDM4, Antibody; Anti-PRDM4 Antibody Picoband; eva-1 homolog A, regulator of programmed cell death; Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24; anti-PRDM4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-PRDM4 antibody
ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E coli-derived human PRDM4 recombinant protein (Position: E87-R405).
Subcellular Localization
Endoplasmic reticulum membrane, Single-pass membrane protein, Lysosome membrane
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
IF (Immunofluorescence)
(Figure 2. IF analysis of PRDM4 using anti-PRDM4 antibody (AAA127737).PRDM4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PRDM4 Antibody (AAA127737) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IF (Immunofluorescence)
(Figure 2. IF analysis of PRDM4 using anti-PRDM4 antibody (AAA127737).PRDM4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PRDM4 Antibody (AAA127737) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
WB (Western Blot)
(Figure 1. Western blot analysis of PRDM4 using anti-PRDM4 antibody (AAA127737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human COLO320 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM4 antigen affinity purified polyclonal antibody (#AAA127737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDM4 at approximately 88 kDa. The expected band size for PRDM4 is at 88 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of PRDM4 using anti-PRDM4 antibody (AAA127737).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human COLO320 whole cell lysates,Lane 2: human RT4 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse heart tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM4 antigen affinity purified polyclonal antibody (#AAA127737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDM4 at approximately 88 kDa. The expected band size for PRDM4 is at 88 kDa.)
Related Product Information for anti-PRDM4 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-PRDM4 antibody
References
1. Briknarova, K., Atwater, D. Z., Glicken, J. M., Maynard, S. J., Ness, T. E. The PR/SET domain in PRDM4 is preceded by a zinc knuckle. Proteins 79: 2341-2345, 2011.2. Gross, M. B. Personal Communication. Baltimore, Md. 9/2/2015.3. Yang, X.-H., Huang, S. PFM1 (PRDM4), a new member of the PR-domain family, maps to a tumor suppressor locus on human chromosome 12q23-q24.1. Genomics 61: 319-325, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
801
NCBI Official Full Name
PR domain zinc finger protein 4
NCBI Official Synonym Full Names
PR domain containing 4
NCBI Official Symbol
PRDM4
NCBI Official Synonym Symbols
PFM1
NCBI Protein Information
PR domain zinc finger protein 4; PR domain-containing protein 4; PR-domain zinc-finger protein PFM1
UniProt Protein Name
PR domain zinc finger protein 4
UniProt Gene Name
PRDM4
UniProt Synonym Gene Names
PFM1
UniProt Entry Name
PRDM4_HUMAN
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Product Notes
The PRDM4 prdm4 (Catalog #AAA127737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRDM4 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PRDM4 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PRDM4 prdm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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