Rabbit PRSS27 Polyclonal Antibody | anti-PRSS27 antibody

Anti-PRSS27 Antibody Picoband

Cat. Num. AAA127821

• Gene Names: PRSS27; MPN; CAPH2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: PRSS27, Antibody; Anti-PRSS27 Antibody Picoband; Follicular dendritic cell secreted peptide; RNA-binding protein 47; RNA-binding motif protein 47; RBM47; anti-PRSS27 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• Applicable Applications for anti-PRSS27 antibody: ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
• Immunogen: E coli-derived human PRSS27 recombinant protein (Position: A23-K290).
• Subcellular Localization: Nucleus
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
• Cross Reactivity: No cross-reactivity with other proteins
• Protein Function: Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.
• Preparation and Storage: At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 2. Flow Cytometry analysis of JK cells using anti-PRSS27 antibody (AAA127821).Overlay histogram showing JK cells stained with AAA127821 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS27 Antibody (AAA127821, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot)

(Figure 1. Western blot analysis of PRSS27 using anti-PRSS27 antibody (AAA127821).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat stomach tissue lysates,Lane 2: mouse pancreas tissue lysates,Lane 3: mouse MFC whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS27 antigen affinity purified polyclonal antibody (#AAA127821) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRSS27 at approximately 32 kDa. The expected band size for PRSS27 is at 28,32 kDa.)

Related Product Information for anti-PRSS27 antibody

The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Categories/Family for anti-PRSS27 antibody

Primary Antibodies; Chromatin Binding Proteins; DNA/RNA Binding; Epigenetics and Nuclear Signaling

References

1. Bhagwandin, V. J., Hau, L. W.-T., Mallen-St. Clair, J., Wolters, P. J., Caughey, G. H. Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas. J. Biol. Chem. 278: 3363-3371, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 971825955
• NCBI GeneID: 83886
• NCBI Accession #: NP_001305324.1
• NCBI GenBank Nucleotide #: NM_001318395.1
• UniProt Accession #: Q9BQR3
• Molecular Weight: 31,940 Da
• NCBI Official Full Name: serine protease 27 isoform 2
• NCBI Official Synonym Full Names: protease, serine 27
• NCBI Official Symbol: PRSS27
• NCBI Official Synonym Symbols: MPN; CAPH2
• NCBI Protein Information: serine protease 27
• UniProt Protein Name: Serine protease 27
• UniProt Gene Name: PRSS27
• UniProt Synonym Gene Names: MPN
• UniProt Entry Name: PRS27_HUMAN