Rabbit anti-Human PSMA1 Polyclonal Antibody | anti-PSMA1 antibody

PSMA1 Antibody

Cat. Num. AAA233277

• Gene Names: PSMA1; NU; HC2; PROS30; HEL-S-275
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: PSMA1, Antibody; PSMA1 Antibody; Proteasome subunit alpha type-1; 30 kDa prosomal protein; PROS-30; Macropain subunit C2; Multicatalytic endopeptidase complex subunit C2; Proteasome component C2; Proteasome nu chain; PSMA1; HC2; NU; PROS30; PSC2; anti-PSMA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 263

• Applicable Applications for anti-PSMA1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Proteasome subunit alpha type-1 protein (163-263AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating PSMA1 in K562 whole cell lysateLane 1: Rabbit control IgG (1ug)instead of AAA233277 in K562 whole cell lysate. For western blotting,a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)Lane 2: AAA233277 (8ug)+ K562 whole cell lysate (500ug)Lane 3: K562 whole cell lysate (10ug))

IF (Immunofluorescence)

(Immunofluorescent analysis of PC3 cells using AAA233277 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemisry)

(Immunohistochemistry of paraffin-embedded human colon cancer using AAA233277 at dilution of 1:100)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human cervical cancer using AAA233277 at dilution of 1:100)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate,PC3 whole cell lysate,K562 whole cell lysateAll lanes: PSMA1 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 30,31 KDaObserved band size: 30 KDa)

Related Product Information for anti-PSMA1 antibody

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 4506179
• NCBI GeneID: 5682
• NCBI Accession #: NP_002777.1
• NCBI GenBank Nucleotide #: NM_002786.3
• UniProt Accession #: P25786; Q53YE8; Q9BRV9; A8K400
• Molecular Weight: 30,239 Da
• NCBI Official Full Name: proteasome subunit alpha type-1 isoform 2
• NCBI Official Synonym Full Names: proteasome subunit alpha 1
• NCBI Official Symbol: PSMA1
• NCBI Official Synonym Symbols: NU; HC2; PROS30; HEL-S-275
• NCBI Protein Information: proteasome subunit alpha type-1
• UniProt Protein Name: Proteasome subunit alpha type-1
• UniProt Gene Name: PSMA1
• UniProt Synonym Gene Names: HC2; NU; PROS30; PSC2; PROS-30