FCM/FACS (Flow Cytometry)
(PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PTPRC Polyclonal Antibody | anti-PTPRC antibody
PTPRC Antibody (N-term)
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PTPRC, Antibody; PTPRC Antibody (N-term); Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; L-CA; T200; CD45; PTPRC; anti-PTPRC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PTPRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the N-terminal region of human PTPRC.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.3 (varies by lot)
Sequence Positions
336-364
Sequence Length
1,143
Applicable Applications for anti-PTPRC antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PTPRC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(PTPRC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRC antibody detected the PTPRC protein (arrow).)
WB (Western Blot)
(PTPRC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PTPRC antibody detected the PTPRC protein (arrow).)
Related Product Information for anti-PTPRC antibody
The protein encoded by this gene is a member of the
protein tyrosine phosphatase (PTP) family. PTPs are known to be
signaling molecules that regulate a variety of cellular processes
including cell growth, differentiation, mitotic cycle, and
oncogenic transformation. This PTP contains an extracellular
domain, a single transmembrane segment and two tandem
intracytoplasmic catalytic domains, and thus belongs to receptor
type PTP. This gene is specifically expressed in hematopoietic
cells. This PTP has been shown to be an essential regulator of T-
and B-cell antigen receptor signaling. It functions through either
direct interaction with components of the antigen receptor
complexes, or by activating various Src family kinases required for
the antigen receptor signaling. This PTP also suppresses JAK
kinases, and thus functions as a regulator of cytokine receptor
signaling. Four alternatively spliced transcripts variants of this
gene, which encode distinct isoforms, have been reported. [provided
by RefSeq].
protein tyrosine phosphatase (PTP) family. PTPs are known to be
signaling molecules that regulate a variety of cellular processes
including cell growth, differentiation, mitotic cycle, and
oncogenic transformation. This PTP contains an extracellular
domain, a single transmembrane segment and two tandem
intracytoplasmic catalytic domains, and thus belongs to receptor
type PTP. This gene is specifically expressed in hematopoietic
cells. This PTP has been shown to be an essential regulator of T-
and B-cell antigen receptor signaling. It functions through either
direct interaction with components of the antigen receptor
complexes, or by activating various Src family kinases required for
the antigen receptor signaling. This PTP also suppresses JAK
kinases, and thus functions as a regulator of cytokine receptor
signaling. Four alternatively spliced transcripts variants of this
gene, which encode distinct isoforms, have been reported. [provided
by RefSeq].
Product Categories/Family for anti-PTPRC antibody
References
Heyd, F., et al. Mol. Cell 40(1):126-137(2010)
Wu, Z., et al. J. Immunol. 185(1):231-238(2010)
Cui, J., et al. Arthritis Rheum. 62(7):1849-1861(2010)
Booth, N.J., et al. J. Immunol. 184(8):4317-4326(2010)
Capitanescu, B., et al. Rom J Morphol Embryol 51(1):49-54(2010)
Wu, Z., et al. J. Immunol. 185(1):231-238(2010)
Cui, J., et al. Arthritis Rheum. 62(7):1849-1861(2010)
Booth, N.J., et al. J. Immunol. 184(8):4317-4326(2010)
Capitanescu, B., et al. Rom J Morphol Embryol 51(1):49-54(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147254
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The PTPRC ptprc (Catalog #AAA289482) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 336-364. The PTPRC Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPRC can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PTPRC ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPRC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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