Mouse anti-Human CD45RB Monoclonal Antibody | anti-PTPRC antibody

CD45RB (B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62690

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: CD45RB, Antibody; CD45RB (B-Cell Marker) Mouse Monoclonal Antibody; B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein; anti-PTPRC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: PTPRC/1147
• Specificity: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1306

• Applicable Applications for anti-PTPRC antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: Recombinant full-length human CD45RB protein
• Hu-Chromosome Location: 1q31.3
• Positive Control: Ramos, U-698, or GA-10 cells. Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

WB (Western Blot)

(Western Blot Analysis of Daudi Cell Lysate using CD45RB Monoclonal Antibody (PTPRC/1147).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RB Monoclonal Antibody (PTPRC/1147).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymphoma stained with CD45RB Monoclonal Antibody (PTPRC/1147).)

References

Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5. Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NM_002838.4
• UniProt Accession #: P08575; Q16614; Q9H0Y6; A8K7W6
• Molecular Weight: 180-220kDa
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN