FCM/FACS (Flow Cytometry)
(RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human RAD17 Polyclonal Antibody | anti-RAD17 antibody
RAD17 Antibody (Center)
Gene Names
RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RAD17, Antibody; RAD17 Antibody (Center); Cell cycle checkpoint protein RAD17; hRad17; RF-C/activator 1 homolog; RAD17; R24L; anti-RAD17 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the Central region of human RAD17.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Positions
218-246
Sequence Length
584
Applicable Applications for anti-RAD17 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RAD17 Antibody (Center) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(RAD17 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).)
WB (Western Blot)
(RAD17 Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RAD17 antibody detected the RAD17 protein (arrow).)
Related Product Information for anti-RAD17 antibody
The protein encoded by this gene is highly similar to the
gene product of Schizosaccharomyces pombe rad17, a cell cycle
checkpoint gene required for cell cycle arrest and DNA damage
repair in response to DNA damage. This protein shares strong
similarity with DNA replication factor C (RFC), and can form a
complex with RFCs. This protein binds to chromatin prior to DNA
damage and is phosphorylated by the checkpoint kinase ATR following
damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein
complex onto chromatin after DNA damage, which may be required for
its phosphorylation. The phosphorylation of this protein is
required for the DNA-damage-induced cell cycle G2 arrest, and is
thought to be a critical early event during checkpoint signaling in
DNA-damaged cells. Eight alternatively spliced transcript variants
of this gene, which encode four distinct proteins, have been
reported. Two pseudogenes, located on chromosomes 7 and 13, have
been identified.
gene product of Schizosaccharomyces pombe rad17, a cell cycle
checkpoint gene required for cell cycle arrest and DNA damage
repair in response to DNA damage. This protein shares strong
similarity with DNA replication factor C (RFC), and can form a
complex with RFCs. This protein binds to chromatin prior to DNA
damage and is phosphorylated by the checkpoint kinase ATR following
damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein
complex onto chromatin after DNA damage, which may be required for
its phosphorylation. The phosphorylation of this protein is
required for the DNA-damage-induced cell cycle G2 arrest, and is
thought to be a critical early event during checkpoint signaling in
DNA-damaged cells. Eight alternatively spliced transcript variants
of this gene, which encode four distinct proteins, have been
reported. Two pseudogenes, located on chromosomes 7 and 13, have
been identified.
Product Categories/Family for anti-RAD17 antibody
References
Zhang, L., et al. EMBO J. 29(10):1726-1737(2010)
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Beretta, G.L., et al. Cancer Lett. 266(2):194-202(2008)
Zhao, M., et al. Head Neck 30(1):35-42(2008)
Rodriguez-Bravo, V., et al. Cancer Res. 66(17):8672-8679(2006)
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Beretta, G.L., et al. Cancer Lett. 266(2):194-202(2008)
Zhao, M., et al. Head Neck 30(1):35-42(2008)
Rodriguez-Bravo, V., et al. Cancer Res. 66(17):8672-8679(2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77055
NCBI Official Full Name
cell cycle checkpoint protein RAD17 isoform 1
NCBI Official Synonym Full Names
RAD17 checkpoint clamp loader component
NCBI Official Symbol
RAD17
NCBI Official Synonym Symbols
CCYC; R24L; RAD24; HRAD17; RAD17SP
NCBI Protein Information
cell cycle checkpoint protein RAD17
UniProt Protein Name
Cell cycle checkpoint protein RAD17
UniProt Gene Name
RAD17
UniProt Synonym Gene Names
R24L; hRad17
UniProt Entry Name
RAD17_HUMAN
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Product Notes
The RAD17 rad17 (Catalog #AAA289553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 218-246. The RAD17 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAD17 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RAD17 rad17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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