WB (Western Blot)
(Detection of human Rad17, Phospho (S645) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Rad17, Phospho (S645) antibody AAA210790 (lot AAA210790-2) used for WB at 0.4 ug/ml. To examine total Rad17, the membrane was reblotted with goat anti-Rad17 antibody BL239 (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
Rabbit anti-Human, Mouse Rad17 Polyclonal Antibody | anti-Rad17 antibody
Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Rad17, Antibody; Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified; Rad17, Phospho (S645); RAD17 homolog; RAD17SP; RAD24; RF-C/activator 1 homolog; Rad17-like protein; R24L; HRAD17; cell cycle checkpoint protein RAD17; cell cycle checkpoint protein (RAD17); CCYC; RF-C activator 1 homolog; RAD1 homolog; Cell cycle checkpoint protein RAD17; RAD17 checkpoint clamp loader component; anti-Rad17 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Rad17 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Surrounding serine 645
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Rad17, Phospho (S645) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Rad17, Phospho (S645) antibody AAA210790 (lot AAA210790-2) used for WB at 0.4 ug/ml. To examine total Rad17, the membrane was reblotted with goat anti-Rad17 antibody BL239 (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
WB (Western Blot)
(Detection of human Rad17, Phospho (S645) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Rad17, Phospho (S645) antibody AAA210790 (lot AAA210790-2) used for WB at 0.4 ug/ml. To examine total Rad17, the membrane was reblotted with goat anti-Rad17 antibody BL239 (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).)
IHC (Immunohiostchemistry)
(Detection of mouse Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of mouse Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Phospho Rad17 (S645) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Phospho Rad17 (S645) (Cat. No. AAA210790 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Rad17 antibody
Rad17 is a cell cycle checkpoint protein that associates with the RFC complex to recruit the 9-1-1 complex (Rad9, Hus1, Rad1) to sites of DNA damage. At DNA lesions, Rad17 and RFC aid in the activation of Chk2 by ATM and ATRIP to trigger the checkpoint signaling cascade for G2 arrest.
Product Categories/Family for anti-Rad17 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Rad17 rad17 (Catalog #AAA210790) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho Rad17 (S645) Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rad17 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Rad17 rad17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.