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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.)
Rabbit Rad21 Polyclonal Antibody | anti-Rad21 antibody
Rad21 Polyclonal Antibody
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
Rad21; Polyclonal Antibody; Rad21 Polyclonal Antibody; CDLS4; HR21; HRAD21; MCD1; NXP1; SCC1; hHR21; anti-Rad21 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-Rad21 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
Immunogen
Recombinant fusion protein of human Rad21
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug Rad21 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using Rad21 Polyclonal Antibody at a dilution of 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using Rad21 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse stomach using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human colon carcinoma using Rad21 Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of C6 cells using Rad21 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using Rad21 Polyclonal Antibody at 1:1000 dilution.)
Related Product Information for anti-Rad21 antibody
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere regionuggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]
Product Categories/Family for anti-Rad21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71690 MW
NCBI Official Full Name
double-strand-break repair protein rad21 homolog
NCBI Official Synonym Full Names
RAD21 cohesin complex component
NCBI Official Symbol
RAD21
NCBI Official Synonym Symbols
HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
NCBI Protein Information
double-strand-break repair protein rad21 homolog
UniProt Protein Name
Double-strand-break repair protein rad21 homolog
UniProt Gene Name
RAD21
UniProt Synonym Gene Names
; hHR21; NXP-1
Similar Products
Product Notes
The Rad21 rad21 (Catalog #AAA22310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rad21 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rad21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the Rad21 rad21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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