WB (Western Blot)
(Detection of mouse Rad21 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse Rad21 Polyclonal Antibody | anti-RAD21 antibody
Rabbit anti-Rad21 Antibody, Affinity Purified
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Rad21, Antibody; Rabbit anti-Rad21 Antibody, Affinity Purified; SCC1; SCC1 homolog; RAD21 homolog; protein involved in DNA double-strand break repair; NXP-1; NXP1; nuclear matrix protein 1; CDLS4; MCD1; kleisin; HRAD21; HR21; hHR21; double-strand-break repair protein rad21 homolog; sister chromatid cohesion 1; MGS; Double-strand-break repair protein rad21 homolog; RAD21 cohesin complex component; anti-RAD21 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RAD21 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 581 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse Rad21 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse Rad21 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Rad21 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Rad21 by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, MCF-7, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (AAA210751 lot 10). For blotting immunoprecipitated Rad21, AAA210751 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody (AAA210751 lot 11) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (AAA210751 lot 10). For blotting immunoprecipitated Rad21, AAA210751 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-RAD21 antibody
Rad21, also called SCC1 (sister chromatid cohesion protein 1), is part of the mitotic cohesin complex that consist of SMC1, SMC3, SCC3 and SCC1. The cohesin complex is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination. Rad21 has been demonstrated to be cleaved by caspase proteins to trigger sister chromatid separation and during apoptotic signaling.
Product Categories/Family for anti-RAD21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71690 MW
NCBI Official Full Name
double-strand-break repair protein rad21 homolog
NCBI Official Synonym Full Names
RAD21 cohesin complex component
NCBI Official Symbol
RAD21
NCBI Official Synonym Symbols
HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
NCBI Protein Information
double-strand-break repair protein rad21 homolog
UniProt Protein Name
Double-strand-break repair protein rad21 homolog
UniProt Gene Name
RAD21
UniProt Synonym Gene Names
; hHR21; NXP-1
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Product Notes
The RAD21 rad21 (Catalog #AAA210751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Rad21 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rad21 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RAD21 rad21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rad21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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