Rabbit anti-Human, Mouse RAN Polyclonal Antibody | anti-RAN antibody

Rabbit anti-RAN Antibody, Affinity Purified

Cat. Num. AAA212604

• Gene Names: RAN; TC4; Gsp1; ARA24
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: RAN, Antibody; Rabbit anti-RAN Antibody, Affinity Purified; OK/SW-cl.81; TC4; ras-related nuclear protein; RanGTPase; member RAS oncogene family; GTP-binding nuclear protein Ran; GTPase Ran; Gsp1; ARA24; androgen receptor-associated protein 24; ras-like protein TC4; guanosine triphosphatase Ran; RAN, member RAS oncogene family; anti-RAN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-RAN antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Rat, Zebrafish, X. laevis, X. tropicalis, Chicken, Bovine, Dog, Orangutan
• Immunogen: Between 166 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human RAN by western blot. Samples: Whole cell lysate (2 ug) from HeLa, Hep-G2, Jurkat, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAN antibody (AAA212604 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

WB (Western Blot)

(Detection of mouse RAN by western blot. Samples: Whole cell lysate (2 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAN antibody (AAA212604 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IP (Immunoprecipitation)

(Detection of human RAN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAN antibody (AAA212604 lot 2) used for IP at 6 ug per reaction. RAN was also immunoprecipitated by a previous lot of this antibody (AAA212604 lot 1) and a another antibody against a different epitope of RAN . For blotting immunoprecipitated RAN, AAA212604 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry)

(Detection of human RAN by immunohistochemistry. Samples: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RAN (AAA212604 lot 2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human RAN by immunohistochemistry. Samples: FFPE section of human breast carcinoma. Antibody:Affinity purified rabbit anti-RAN (AAA212604 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

Related Product Information for anti-RAN antibody

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor [taken from NCBI Entrez Gene (Gene ID: 5901)].

Product Categories/Family for anti-RAN antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5453555
• NCBI GeneID: 5901
• NCBI Accession #: NP_006316.1
• NCBI GenBank Nucleotide #: NM_006325.3
• UniProt Accession #: P62826; P17080; P28746; P28747; Q6IPB2; Q86V08; Q8NI90; Q9CSP3; Q9CWI7; Q9CZA2; Q9UDJ5; A8K3Z8; AAB24940
• Molecular Weight: 24,423 Da
• NCBI Official Full Name: GTP-binding nuclear protein Ran
• NCBI Official Synonym Full Names: RAN, member RAS oncogene family
• NCBI Official Symbol: RAN
• NCBI Official Synonym Symbols: TC4; Gsp1; ARA24
• NCBI Protein Information: GTP-binding nuclear protein Ran; RanGTPase; GTPase Ran; OK/SW-cl.81; ras-like protein TC4; member RAS oncogene family; ras-related nuclear protein; guanosine triphosphatase Ran; androgen receptor-associated protein 24
• UniProt Protein Name: GTP-binding nuclear protein Ran
• UniProt Gene Name: RAN
• UniProt Synonym Gene Names: ARA24
• UniProt Entry Name: RAN_HUMAN