WB (Western Blot)
(All lanes : Anti-RAPGEF1 Antibody (Center) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human RAPGEF1 Polyclonal Antibody | anti-RAPGEF1 antibody
RAPGEF1 Antibody (Center)
Gene Names
RAPGEF1; C3G; GRF2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RAPGEF1, Antibody; RAPGEF1 Antibody (Center); Rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; Guanine nucleotide-releasing factor 2; Protein C3G; RAPGEF1; GRF2; anti-RAPGEF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RAPGEF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 536-569 amino acids from the Central region of human RAPGEF1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1077
Applicable Applications for anti-RAPGEF1 antibody
ELISA, WB (Western Blot)
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-RAPGEF1 Antibody (Center) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-RAPGEF1 Antibody (Center) at 1:1000-1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RAPGEF1 Antibody (Center)at 1:2000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-RAPGEF1 Antibody (Center)at 1:2000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-RAPGEF1 antibody
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Product Categories/Family for anti-RAPGEF1 antibody
References
Tanaka S.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994).
Knudsen B.,et al.J. Biol. Chem. 269:32781-32787(1994).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Humphray S.J.,et al.Nature 429:369-374(2004).
Matsuda M.,et al.J. Biol. Chem. 271:14468-14472(1996).
Knudsen B.,et al.J. Biol. Chem. 269:32781-32787(1994).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Humphray S.J.,et al.Nature 429:369-374(2004).
Matsuda M.,et al.J. Biol. Chem. 271:14468-14472(1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120548
NCBI Official Full Name
rap guanine nucleotide exchange factor 1 isoform a
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol
RAPGEF1
NCBI Official Synonym Symbols
C3G; GRF2
NCBI Protein Information
rap guanine nucleotide exchange factor 1
UniProt Protein Name
Rap guanine nucleotide exchange factor 1
UniProt Gene Name
RAPGEF1
UniProt Synonym Gene Names
GRF2
UniProt Entry Name
RPGF1_HUMAN
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Product Notes
The RAPGEF1 rapgef1 (Catalog #AAA286501) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAPGEF1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAPGEF1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RAPGEF1 rapgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAPGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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