WB (Western Blot)
(Detection of human RNASEH2A by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody AAA212521 (lot AAA212521-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human RNASEH2A Polyclonal Antibody | anti-RNASEH2A antibody
Rabbit anti-RNASEH2A Antibody, Affinity Purified
Gene Names
RNASEH2A; AGS4; JUNB; RNHL; RNHIA; RNASEHI
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RNASEH2A, Antibody; Rabbit anti-RNASEH2A Antibody, Affinity Purified; RNase H(35); RNASEHI; THSD8; RNHIA; RNase HI large subunit; RNase H2 subunit A; ribonuclease HI large subunit; ribonuclease H2, large subunit; ribonuclease H2 subunit A; AGS4; JUNB; ribonuclease HI subunit A; aicardi-Goutieres syndrome 4 protein; RNHL; Ribonuclease H2 subunit A; anti-RNASEH2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RNASEH2A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 249 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human RNASEH2A by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody AAA212521 (lot AAA212521-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human RNASEH2A by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody AAA212521 (lot AAA212521-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RNASEH2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody AAA212521 (lot AAA212521-1) used for IP at 6 ug/mg lysate. RNASEH2A was also immunoprecipitated by rabbit anti-RNASEH2A antibodies A304-148A and A304-150A. For blotting immunoprecipitated RNASEH2A, AAA212521 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RNASEH2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RNASEH2A antibody AAA212521 (lot AAA212521-1) used for IP at 6 ug/mg lysate. RNASEH2A was also immunoprecipitated by rabbit anti-RNASEH2A antibodies A304-148A and A304-150A. For blotting immunoprecipitated RNASEH2A, AAA212521 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-RNASEH2A antibody
RNASEH2A is the catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. RNASEH2A participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. It also mediates the excision of single ribonucleotides from DNA:RNA duplexes [taken from NCBI Entrez Gene (Gene ID: 10535)].
Product Categories/Family for anti-RNASEH2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,395 Da
NCBI Official Full Name
ribonuclease H2 subunit A
NCBI Official Synonym Full Names
ribonuclease H2, subunit A
NCBI Official Symbol
RNASEH2A
NCBI Official Synonym Symbols
AGS4; JUNB; RNHL; RNHIA; RNASEHI
NCBI Protein Information
ribonuclease H2 subunit A; RNase H(35); RNase H2 subunit A; RNase HI large subunit; aicardi-Goutieres syndrome 4 protein; ribonuclease H2, large subunit; ribonuclease HI large subunit; ribonuclease HI subunit A; ribonuclease HI, large subunit
UniProt Protein Name
Ribonuclease H2 subunit A
UniProt Gene Name
RNASEH2A
UniProt Synonym Gene Names
RNASEHI; RNHIA; RNase H2 subunit A; AGS4; RNase HI large subunit
UniProt Entry Name
RNH2A_HUMAN
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Product Notes
The RNASEH2A rnaseh2a (Catalog #AAA212521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RNASEH2A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNASEH2A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RNASEH2A rnaseh2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNASEH2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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