WB (Western Blot)
(Detection of human Roquin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Roquin antibody AAA210993 (lot AAA210993-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Roquin was also immunoprecipitated by a previous lot (lot AAA210993-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
Rabbit anti-Human, Mouse Roquin Polyclonal Antibody | anti-RC3H1 antibody
Rabbit anti-Roquin Antibody, Affinity Purified
Gene Names
RC3H1; RNF198; ROQUIN; KIAA2025; RP5-1198E17.5
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Roquin, Antibody; Rabbit anti-Roquin Antibody, Affinity Purified; roquin-1; RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198; RNF198; ROQUIN; probable E3 ubiquitin-protein ligase Roquin; Roquin-1; ring finger and CCCH-type domains 1; anti-RC3H1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RC3H1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 500 and 550
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Roquin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Roquin antibody AAA210993 (lot AAA210993-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Roquin was also immunoprecipitated by a previous lot (lot AAA210993-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
WB (Western Blot)
(Detection of human Roquin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Roquin antibody AAA210993 (lot AAA210993-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Roquin was also immunoprecipitated by a previous lot (lot AAA210993-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).)
WB (Western Blot)
(Detection of mouse Roquin by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody AAA210993 (lot AAA210993-1) used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse Roquin by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody AAA210993 (lot AAA210993-1) used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-RC3H1 antibody
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Roquin is a member of the RING type E3 ubiquitin ligases. Roquin possesses a CCCH zinc-finger found in RNA-binding proteins and is implicated to play a role in the development of autoimmune disease.
Product Categories/Family for anti-RC3H1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125,736 Da
NCBI Official Full Name
roquin
NCBI Official Synonym Full Names
ring finger and CCCH-type domains 1
NCBI Official Symbol
RC3H1
NCBI Official Synonym Symbols
RNF198; ROQUIN; KIAA2025; RP5-1198E17.5
NCBI Protein Information
roquin; OTTHUMP00000035392; OTTHUMP00000035394; RING finger protein 198; RING finger and C3H zinc finger protein 1; ring finger and CCCH-type zinc finger domains 1
UniProt Protein Name
Roquin-1
UniProt Gene Name
RC3H1
UniProt Synonym Gene Names
KIAA2025; RNF198; Roquin
UniProt Entry Name
RC3H1_HUMAN
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Product Notes
The RC3H1 rc3h1 (Catalog #AAA210993) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Roquin Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Roquin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RC3H1 rc3h1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Roquin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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