IP (Immunoprecipitation)
(Detection of human RSK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RSK1 antibody AAA211880 used for IP at 3 ug/mg lysate. RSK1 was also immunoprecipitated by rabbit anti-RSK1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RSK1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human RSK1 Polyclonal Antibody | anti-RSK1 antibody
Rabbit anti-RSK1 Antibody, Affinity Purified
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
RSK1, Antibody; Rabbit anti-RSK1 Antibody, Affinity Purified; p90Rsk; p90S6K; S6K-alpha 1; RSK-1; RSK1; RSK; ribosomal S6 kinase 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase alpha-1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); p90RSK1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK1A; MAPKAPK-1a; S6K-alpha-1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase A1; anti-RSK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RSK1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 375 and 425
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human RSK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RSK1 antibody AAA211880 used for IP at 3 ug/mg lysate. RSK1 was also immunoprecipitated by rabbit anti-RSK1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RSK1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human RSK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RSK1 antibody AAA211880 used for IP at 3 ug/mg lysate. RSK1 was also immunoprecipitated by rabbit anti-RSK1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RSK1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human RSK1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RSK1 (Cat. No. AAA211880 Lot1) used at a dilution of 1:400 (2.5 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
IHC (Immunohistochemistry)
(Detection of human RSK1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RSK1 (Cat. No. AAA211880 Lot1) used at a dilution of 1:400 (2.5 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100.)
Related Product Information for anti-RSK1 antibody
Ribosomal protein S6 kinase alpha-1 (RSK1) is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. RSK1 kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signaling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation [taken from NCBI Entrez Gene (GeneID: 6195)].
Product Categories/Family for anti-RSK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,698 Da
NCBI Official Full Name
ribosomal protein S6 kinase alpha-1 isoform a
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol
RPS6KA1
NCBI Official Synonym Symbols
RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
NCBI Protein Information
ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinas
UniProt Protein Name
Ribosomal protein S6 kinase alpha-1
UniProt Gene Name
RPS6KA1
UniProt Synonym Gene Names
MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1
UniProt Entry Name
KS6A1_HUMAN
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Product Notes
The RSK1 rps6ka1 (Catalog #AAA211880) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RSK1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RSK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the RSK1 rps6ka1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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