WB (Western Blot)
(Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody AAA213090 (lot AAA213090-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse RTCB/C22orf28/FAAP Polyclonal Antibody | anti-RTCB/C22orf28/FAAP antibody
Rabbit anti-RTCB/C22orf28/FAAP Antibody, Affinity Purified
Gene Names
RTCB; FAAP; HSPC117; C22orf28; DJ149A16.6
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
RTCB/C22orf28/FAAP, Antibody; Rabbit anti-RTCB/C22orf28/FAAP Antibody, Affinity Purified; focal adhesion-associated protein; HSPC117; FAAP; DJ149A16.6; C22orf28; ankyrin repeat domain 54; tRNA-splicing ligase RtcB homolog; RNA 2',3'-cyclic phosphate and 5'-OH ligase; anti-RTCB/C22orf28/FAAP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-RTCB/C22orf28/FAAP antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Zebrafish, X. tropicalis, Bovine, Pig
Immunogen
Between 455 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody AAA213090 (lot AAA213090-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse RTCB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RTCB antibody AAA213090 (lot AAA213090-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RTCB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RTCB antibody AAA213090 (lot AAA213090-1) used for IP at 6 ug per reaction. RTCB was also immunoprecipitated by rabbit anti-RTCB antibody For blotting immunoprecipitated RTCB, AAA213090 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human RTCB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RTCB antibody AAA213090 (lot AAA213090-1) used for IP at 6 ug per reaction. RTCB was also immunoprecipitated by rabbit anti-RTCB antibody For blotting immunoprecipitated RTCB, AAA213090 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-RTCB/C22orf28/FAAP antibody
RTCB encodes tRNA-splicing ligase RtcB homolog, the catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs. tRNA-splicing ligase RtcB homolog may also act as an RNA ligase with broad substrate specificity, and may function toward other RNAs [taken from UniProtKB (Entry: Q9Y3I0)].
Product Categories/Family for anti-RTCB/C22orf28/FAAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,210 Da
NCBI Official Full Name
tRNA-splicing ligase RtcB homolog
NCBI Official Synonym Full Names
RNA 2',3'-cyclic phosphate and 5'-OH ligase
NCBI Official Symbol
RTCB
NCBI Official Synonym Symbols
FAAP; HSPC117; C22orf28; DJ149A16.6
NCBI Protein Information
tRNA-splicing ligase RtcB homolog
UniProt Protein Name
tRNA-splicing ligase RtcB homolog
UniProt Gene Name
RTCB
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Product Notes
The RTCB/C22orf28/FAAP rtcb (Catalog #AAA213090) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-RTCB/C22orf28/FAAP Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RTCB/C22orf28/FAAP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the RTCB/C22orf28/FAAP rtcb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RTCB/C22orf28/FAAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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