IHC (Immunohistochemisry)
(Fig. 3: Immunohistochemical analysis of CD43 in human spleen tissue using CD43 antibody (Clone: DF-T1) at 10 ug/ml.)
Mouse anti-Human CD43 Monoclonal Antibody | anti-CD43 antibody
Monoclonal Antibody to CD43 (Clone: DF-T1)
Gene Names
SPN; LSN; CD43; GALGP; GPL115
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
PurifiedProtein G Chromatography
Synonyms
CD43, Antibody; Monoclonal Antibody to CD43 (Clone: DF-T1); SPN; CD43; Galactoglycoprotein; Leukocyte sialoglycoprotein; Sialophorin; anti-CD43 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
DF-T1
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
400
Applicable Applications for anti-CD43 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Antigen
CD43
Immunogen Information
Myeloblastic KG1 cells were used as the immunogen for this antibody.
Conjugation
Unconjugated
Preparation and Storage
Store the antibody at 4 degree C; stable for 6 months.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage; store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohistochemisry)
(Fig. 3: Immunohistochemical analysis of CD43 in human spleen tissue using CD43 antibody (Clone: DF-T1) at 10 ug/ml.)
IHC (Immunohistochemisry)
(Fig. 3: Immunohistochemical analysis of CD43 in human spleen tissue using CD43 antibody (Clone: DF-T1) at 10 ug/ml.)
IHC (Immunohiostchemistry)
(Fig. 2: Immunohistochemical analysis of CD43 in human spleen using CD43 antibody (Clone: DF-T1) at 1: 200 dilution.)
IHC (Immunohiostchemistry)
(Fig. 2: Immunohistochemical analysis of CD43 in human spleen using CD43 antibody (Clone: DF-T1) at 1: 200 dilution.)
FCM/FACS (Flow Cytometry)
(Fig.1: Cell Surface flow analysis of anti-hCD43 antibody (36-3009) in PBMC (Lymphocytes gated) using 0.5 ug/10^6 cells. Green represents isotype control; red represents anti-hCD43 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
FCM/FACS (Flow Cytometry)
(Fig.1: Cell Surface flow analysis of anti-hCD43 antibody (36-3009) in PBMC (Lymphocytes gated) using 0.5 ug/10^6 cells. Green represents isotype control; red represents anti-hCD43 antibody. Goat anti-mouse PE conjugated secondary antibody was used.)
Related Product Information for anti-CD43 antibody
This antibody recognizes a 95/115/135kDa (depending upon the extent of glycosylation) surface transmembrane glycoprotein, identified as CD43 (leukosialin, sialophorin, or leukocyte sialoglycoprotein). CD43 is expressed on all thymocytes and T-lymphocytes. Defects in the CD43 molecule are associated with the development of Wiskott-Aldrich syndrome. CD43 is also found in a range of lymphoid and myeloid tumors. Expression of CD43 may be used to identify B-lymphoblastic lymphoma, since the malignant cells in this condition are often CD43 positive. Since CD43 antibody stains granulocytes and their precursors, this antibody may be used as a marker for myeloid tumors. This antibody is also useful in identification and classification of T-cell malignancies.
Product Categories/Family for anti-CD43 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,322 Da
NCBI Official Full Name
leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
SPN
NCBI Official Synonym Symbols
LSN; CD43; GALGP; GPL115
NCBI Protein Information
leukosialin
UniProt Protein Name
Leukosialin
UniProt Gene Name
SPN
UniProt Synonym Gene Names
CD43; GALGP
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Product Notes
The CD43 spn (Catalog #AAA78452) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD43 (Clone: DF-T1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD43 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD43 spn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD43, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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