WB (Western Blot)
(Detection of human CD43 by western blot. Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody AAA212665 (lot AAA212665-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CD43 Polyclonal Antibody | anti-CD43 antibody
Rabbit anti-CD43 Antibody, Affinity Purified
Gene Names
SPN; LSN; CD43; GALGP; GPL115
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CD43, Antibody; Rabbit anti-CD43 Antibody, Affinity Purified; leukosialin; CD antigen CD43; sialophorin (gpL115, leukosialin, CD43); Sialophorin; LSN; leukocyte sialoglycoprotein; GPL115; GALGP; CD43; galactoglycoprotein; Leukosialin; sialophorin; anti-CD43 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CD43 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 350 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD43 by western blot. Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody AAA212665 (lot AAA212665-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CD43 by western blot. Samples: Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody AAA212665 (lot AAA212665-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CD43 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody AAA212665 (lot AAA212665-1) used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43, AAA212665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CD43 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody AAA212665 (lot AAA212665-1) used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43, AAA212665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human CD43 by immunohistochemistry. Sample: FFPE section of human lung cancer infiltrating cells. Antibody: Affinity purified rabbit anti-CD43 (Cat. No. AAA212665 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human CD43 by immunohistochemistry. Sample: FFPE section of human lung cancer infiltrating cells. Antibody: Affinity purified rabbit anti-CD43 (Cat. No. AAA212665 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-CD43 antibody
CD43 is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, CD43 is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune respons [taken from NCBI Entrez Gene (Gene ID: 6693)].
Product Categories/Family for anti-CD43 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,322 Da
NCBI Official Full Name
leukosialin
NCBI Official Synonym Full Names
sialophorin
NCBI Official Symbol
SPN
NCBI Official Synonym Symbols
LSN; CD43; GALGP; GPL115
NCBI Protein Information
leukosialin; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43)
UniProt Protein Name
Leukosialin
UniProt Gene Name
SPN
UniProt Synonym Gene Names
CD43; GALGP
UniProt Entry Name
LEUK_HUMAN
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Product Notes
The CD43 spn (Catalog #AAA212665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD43 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD43 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD43 spn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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