ICC (Immunocytochemistry)
(ICC Staining RYR3 in Lovo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).)
Rabbit RYR3 Polyclonal Antibody | anti-RYR3 antibody
RYR3 Antibody
Gene Names
RYR3; RYR-3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Dot Blot
Purity
Affinity purified.
Synonyms
RYR3, Antibody; RYR3 Antibody; Brain ryanodine receptor-calcium release channelBrain-type ryanodine receptor; Type 3 ryanodine receptor; Ryanodine receptor 3; Brain ryanodine receptor calcium release channel antibody; Brain type ryanodine receptor antibody; Cardiac muscle ryanodine receptor calcium release channel antibody; Cardiac muscle type ryanodine receptor antibody;CCO antibody; Central core disease of muscle antibody; RYR3 antibody; Sarcoplasmic reticulum calcium release channel antibody; Skeletal muscle calcium release channel antibody; Skeletal muscle ryanodine receptor antibody; SKRR antibody; VTSIP antibody.; anti-RYR3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified.
Form/Format
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Sequence Length
4870
Applicable Applications for anti-RYR3 antibody
ELISA, ICC (Immunocytochemistry), IHC (Immunohistochemistry), DB (Dot Blot)
Immunogen
Recombinant protein within Human RYR3 aa 1-120.
Positive control
Lovo, rat brain tissue, human kidney tissue, human placenta tissue, mouse brain tissue.
Conjugate
Non-conjugated
Subcellular location
Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum.
Preparation and Storage
instructionsStore at +4 degree C after thawing. Aliquot store at -20 degree C. Avoid repeated freeze/thaw cycles.
ICC (Immunocytochemistry)
(ICC Staining RYR3 in Lovo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).)
ICC (Immunocytochemistry)
(ICC Staining RYR3 in Lovo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).)
DB (Dot Blot)
(Dot blot analysis of RYR3 on RYR3 recombinant protein. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.)
DB (Dot Blot)
(Dot blot analysis of RYR3 on RYR3 recombinant protein. The primary antibody was used at a 1:2,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.)
Related Product Information for anti-RYR3 antibody
The protein encoded by this gene is a ryanodine receptor, which functions to release calcium from intracellular storage for use in many cellular processes. For example, the encoded protein is involved in skeletal muscle contraction by releasing calcium from the sarcoplasmic reticulum followed by depolarization of T-tubules.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14.8 kDa
NCBI Official Full Name
ryanodine receptor 3 isoform 1
NCBI Official Synonym Full Names
ryanodine receptor 3
NCBI Official Symbol
RYR3
NCBI Official Synonym Symbols
RYR-3
NCBI Protein Information
ryanodine receptor 3
UniProt Protein Name
Ryanodine receptor 3
UniProt Gene Name
RYR3
UniProt Synonym Gene Names
HBRR; RYR-3; RyR3
Similar Products
Product Notes
The RYR3 ryr3 (Catalog #AAA268292) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RYR3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RYR3 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IHC (Immunohistochemistry), DB (Dot Blot). Researchers should empirically determine the suitability of the RYR3 ryr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.