Rabbit anti-Human SELPLG Polyclonal Antibody | anti-SELPLG antibody

SELPLG Antibody (C-term)

Cat. Num. AAA289865

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: SELPLG, Antibody; SELPLG Antibody (C-term); P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162; SELPLG; anti-SELPLG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This SELPLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-405 amino acids from the C-terminal region of human SELPLG.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 379-405
• Sequence Length: 428

• Applicable Applications for anti-SELPLG antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
• Cellular Location: Membrane; Single-pass type I membrane protein.
• Tissue Location: Expressed on neutrophils, monocytes and most lymphocytes
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(SELPLG Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(SELPLG Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SELPLG antibody detected the SELPLG protein (arrow).)

Related Product Information for anti-SELPLG antibody

SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.

Product Categories/Family for anti-SELPLG antibody

Cardiovascular

References

Elmas, E., et al. J. Cardiovasc. Electrophysiol. 21(11):1260-1265(2010)
Luan, S.L., et al. J. Pathol. 222(2):166-179(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Russo, H.M., et al. Circ. Res. 107(3):388-397(2010)
Dick, D.M., et al. Mol. Psychiatry (2010) In press :

NCBI and Uniprot Product Information

• NCBI GI #: 331284238
• NCBI GeneID: 6404
• NCBI Accession #: NP_001193538.1; NP_002997.2
• NCBI GenBank Nucleotide #: NM_001206609.1
• UniProt Accession #: Q14242; Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6
• Molecular Weight: 43201
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN