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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells usingug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using SHMT2 Polyclonal Antibody at a dilution of 1:1000.)
Rabbit anti-Human SHMT2 Polyclonal Antibody | anti-SHMT2 antibody
SHMT2 Polyclonal Antibody
Gene Names
SHMT2; GLYA; SHMT; HEL-S-51e
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
SHMT2; Polyclonal Antibody; SHMT2 Polyclonal Antibody; GLYA; HEL-S-51e; SHMT; serine hydroxymethyltransferase 2; anti-SHMT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-SHMT2 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
IHC: 1:50-1:100
IF1:50-1:200
IP: 1:50-1:200
IF1:50-1:200
IP: 1:50-1:200
Immunogen
Recombinant fusion protein of human SHMT2
Research Area
Cancer, Metabolism, Signal Transduction
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells usingug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using SHMT2 Polyclonal Antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells usingug SHMT2 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using SHMT2 Polyclonal Antibody at a dilution of 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of L929 cells using SHMT2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat spleen using SHMT2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)
Related Product Information for anti-SHMT2 antibody
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has beenuggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-SHMT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
504
NCBI Official Full Name
Serine hydroxymethyltransferase, mitochondrial
NCBI Official Synonym Full Names
serine hydroxymethyltransferase 2 (mitochondrial)
NCBI Official Symbol
SHMT2
NCBI Official Synonym Symbols
GLYA; SHMT; HEL-S-51e
NCBI Protein Information
serine hydroxymethyltransferase, mitochondrial; GLY A+; serine aldolase; serine methylase; threonine aldolase; serine hydroxymethylase; glycine hydroxymethyltransferase; epididymis secretory sperm binding protein Li 51e; glycine auxotroph A, human complem
UniProt Protein Name
Serine hydroxymethyltransferase, mitochondrial
UniProt Gene Name
SHMT2
UniProt Synonym Gene Names
SHMT
UniProt Entry Name
GLYM_HUMAN
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Product Notes
The SHMT2 shmt2 (Catalog #AAA22331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHMT2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SHMT2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). IHC: 1:50-1:100 IF1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the SHMT2 shmt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SHMT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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