IHC (Immunohiostchemistry)
(SIM1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit SIM1 Polyclonal Antibody | anti-SIM1 antibody
SIM1 Antibody (N-term)
Gene Names
SIM1; bHLHe14
Reactivity
Human (Predicted Reactivity: Mouse, Drosophila)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SIM1, Antibody; SIM1 Antibody (N-term); Single-minded homolog 1; Class E basic helix-loop-helix protein 14; bHLHe14; SIM1; BHLHE14; anti-SIM1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Drosophila)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
1-30
Sequence Length
766
Applicable Applications for anti-SIM1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(SIM1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SIM1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term). AAA289942 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term). AAA289942 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-SIM1 antibody
SIM1 and SIM2 genes are Drosophila single-minded (sim)
gene homologs. SIM1 transcript was detected only in fetal kidney
out of various adult and fetal tissues tested. Since the sim gene
plays an important role in Drosophila development and has peak
levels of expression during the period of neurogenesis,it was
proposed that the human SIM gene is a candidate for involvement in
certain dysmorphic features (particularly the facial and skull
characteristics), abnormalities of brain development, and/or mental
retardation of Down syndrome.
gene homologs. SIM1 transcript was detected only in fetal kidney
out of various adult and fetal tissues tested. Since the sim gene
plays an important role in Drosophila development and has peak
levels of expression during the period of neurogenesis,it was
proposed that the human SIM gene is a candidate for involvement in
certain dysmorphic features (particularly the facial and skull
characteristics), abnormalities of brain development, and/or mental
retardation of Down syndrome.
Product Categories/Family for anti-SIM1 antibody
References
Ghoussaini, M., et al. Obesity (Silver Spring) 18(8):1670-1675(2010)
Tolson, K.P., et al. J. Neurosci. 30(10):3803-3812(2010)
Traurig, M., et al. Diabetes 58(7):1682-1689(2009)
Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009) :
Hung, C.C., et al. Int J Obes (Lond) 31(3):429-434(2007)
Tolson, K.P., et al. J. Neurosci. 30(10):3803-3812(2010)
Traurig, M., et al. Diabetes 58(7):1682-1689(2009)
Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009) :
Hung, C.C., et al. Int J Obes (Lond) 31(3):429-434(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
85515
NCBI Official Full Name
single-minded homolog 1
NCBI Official Synonym Full Names
single-minded family bHLH transcription factor 1
NCBI Official Symbol
SIM1
NCBI Official Synonym Symbols
bHLHe14
NCBI Protein Information
single-minded homolog 1
UniProt Protein Name
Single-minded homolog 1
UniProt Gene Name
SIM1
UniProt Synonym Gene Names
BHLHE14; bHLHe14
UniProt Entry Name
SIM1_HUMAN
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Product Notes
The SIM1 sim1 (Catalog #AAA289942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The SIM1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Drosophila) and may cross-react with other species as described in the data sheet. AAA Biotech's SIM1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the SIM1 sim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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