WB (Western Blot)
(Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody AAA211111 (lot AAA211111-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Sin3A Polyclonal Antibody | anti-Sin3A antibody
Rabbit anti-Sin3A Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Sin3A, Antibody; Rabbit anti-Sin3A Antibody, Affinity Purified; transcriptional regulator, SIN3A; WITKOS; transcriptional co-repressor Sin3A; SIN3 homolog A, transcription regulator; paired amphipathic helix protein Sin3a; histone deacetylase complex subunit Sin3a; transcriptional corepressor Sin3a; Paired amphipathic helix protein Sin3a; SIN3 transcription regulator family member A; anti-Sin3A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Sin3A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody AAA211111 (lot AAA211111-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Sin3A by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Sin3A antibody AAA211111 (lot AAA211111-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody AAA211111 (lot AAA211111-2) used for IP at 6 ug per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot AAA211111-1) and rabbit anti-Sin3A antibodies and A300-726A. For blotting immunoprecipitated Sin3A, AAA211111 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Sin3A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Sin3A antibody AAA211111 (lot AAA211111-2) used for IP at 6 ug per reaction. Sin3A was also immunoprecipitated by a previous lot of this antibody (lot AAA211111-1) and rabbit anti-Sin3A antibodies and A300-726A. For blotting immunoprecipitated Sin3A, AAA211111 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-Sin3A antibody
Sin3A is a part of a histone deacetylase co-repressor complex that functions to stabilize complex members and regulate gene expression through deacetylation of histones. Sin3A acts as a scaffold for a core complex which contains HDAC1, HDAC2, RbAp46, RbAp48, SAP30, RBP1 and p33INHib. Sin3A also associates with the Mad/Max complex to mediate transcriptional repression.
Product Categories/Family for anti-Sin3A antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Sin3A sin3a (Catalog #AAA211111) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Sin3A Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Sin3A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Sin3A sin3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sin3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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