WB (Western Blot)
(SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ?g) probed with SIN3A antibody at a dilution of 1:5,000.)
Rabbit anti-Human, Mouse SIN3A Polyclonal Antibody | anti-SIN3A antibody
SIN3A antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Synonyms
SIN3A, Antibody; SIN3A antibody (pAb); anti-SIN3A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SIN3A antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Immunogen
This SIN3A antibody was raised against a peptide derived from the N-terminus of human SIN3A.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ?g) probed with SIN3A antibody at a dilution of 1:5,000.)
WB (Western Blot)
(SIN3A antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ?g) probed with SIN3A antibody at a dilution of 1:5,000.)
ChIP (Chromatin Immunoprecipitation)
(SIN3A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from TM4 Sertoli cells and 5 ul of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
ChIP (Chromatin Immunoprecipitation)
(SIN3A antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from TM4 Sertoli cells and 5 ul of SIN3A antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Related Product Information for anti-SIN3A antibody
Short Description: Our SIN3A antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples.
Background: SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.
Background: SIN3A (Switch independent 3 homologue) is the mammalian homologue of the yeast protein Sin3. SIN3A is transcriptional co-repressor that is recruited to promoters by sequence-specific DNA binding proteins, such as REST, MXI1 and MAD-MAX. It is part of a large multi-subunit repressor protein complex that also contains HDAC1, HDAC2 and SMRT/NCoR1.
Product Categories/Family for anti-SIN3A antibody
NCBI and Uniprot Product Information
Molecular Weight
155kDa
Similar Products
Product Notes
The SIN3A (Catalog #AAA59940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIN3A antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIN3A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the SIN3A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIN3A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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