IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat Sirtuin 1/SIRT1 Polyclonal Antibody | anti-SIRT1 antibody
Goat anti-Sirtuin 1/SIRT1 (aa577-590) Antibody
Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Sirtuin 1/SIRT1, Antibody; Goat anti-Sirtuin 1/SIRT1 (aa577-590) Antibody; Goat Anti-SIRT1 Antibody; SIRT1; sirtuin 1; SIR2L1; NAD-dependent deacetylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; SIR2-like protein 1; SIR2alpha; hSIR2; hSIRT1; sir2-like 1; sirtuin type 1; SIRT1 antibody; sirtuin 1 antibody; SIR2L1 antibody; NAD-dependent deacetylase sirtuin-1 antibody; OTTHUMP00000198111 antibody; OTTHUMP00000198112 antibody; SIR2-like protein 1 antibody; SIR2alpha antibody; hSIR2 antibody; sir2-like 1 antibody; sirtuin type 1 antibody; Sirtuin 1; Sirtuin 1 / SIRT1 (aa577-590); anti-SIRT1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EKPQEVQTSRNVES
Sequence Length
747
Applicable Applications for anti-SIRT1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohiostchemistry)
((3.8ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
WB (Western Blot)
((0. 05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((0. 05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,066 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform a
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2; SIR2L1; SIR2alpha
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1
UniProt Entry Name
SIR1_HUMAN
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Product Notes
The SIRT1 sirt1 (Catalog #AAA61546) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Sirtuin 1/SIRT1 (aa577-590) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sirtuin 1/SIRT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EKPQEVQTSR NVES. It is sometimes possible for the material contained within the vial of "Sirtuin 1/SIRT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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