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Standard Curve (Sample)

Human Sirtuin 1 (SIRT1) ELISA Kit | SIRT1 elisa kit

Human Sirtuin 1 (SIRT1) ELISA Kit

Gene Names
SIRT1; SIR2; SIR2L1; SIR2alpha
Reactivity
Human
Synonyms
Sirtuin 1 (SIRT1); N/A; Human Sirtuin 1 (SIRT1) ELISA Kit; SIRT1 elisa kit
Ordering
 
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For Research Use Only!
Reactivity
Human
Sequence Length
555
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
Detection Range
0.2-60ng/ml
Sensitivity
0.11ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for SIRT1 elisa kit
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SIRT1 antibody. SIRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SIRT1 Antibody is added and binds to SIRT1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SIRT1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SIRT1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Sirtuin 1 (also known as SIRT1) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
61,066 Da
NCBI Official Full Name
SIRT1 protein
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2; SIR2L1; SIR2alpha
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1

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Product Notes

The Human SIRT1 sirt1 (Catalog #AAA11127) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11127 ELISA Kit recognizes Human SIRT1. It is sometimes possible for the material contained within the vial of "Sirtuin 1 (SIRT1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.