Human Sirtuin 1 ELISA Kit | SIRT1 elisa kit

Human Sirtuin 1 ELISA Kit

Cat. Num. AAA17557

• Gene Names: sirt1; sir2l1; xsirt1; sirtuin
• Reactivity: Human
• Synonyms: Sirtuin 1; N/A; Human Sirtuin 1 ELISA Kit; SIRT1/SIR2alpha/SIR2L1/SIR2-like protein 1/EC 3.5.1/S. cerevisiae; homolog) 1/sirtuin 1; SIRT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SIRT1. No significant cross-reactivity or interference between SIRT1 and analogues was observed.
• Sequence Length: 710
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SIRT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SIRT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for SIRT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-SIRT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SIRT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SIRT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SIRT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148237806
• NCBI GeneID: 100036963
• NCBI Accession #: NP_001091195.1
• NCBI GenBank Nucleotide #: NM_001097726.1
• NCBI Official Full Name: sirtuin 1
• NCBI Official Synonym Full Names: sirtuin 1
• NCBI Official Symbol: sirt1
• NCBI Official Synonym Symbols: sir2l1; xsirt1; sirtuin
• NCBI Protein Information: sirtuin 1