Rabbit SMAD6 Polyclonal Antibody | anti-SMAD6 antibody

Anti-SMAD6 Antibody (aa323-372)

Cat. Num. AAA162306

• Gene Names: SMAD6; AOVD2; MADH6; MADH7; HsT17432
• Reactivity: Human; Predicted: Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SMAD6, Antibody; Anti-SMAD6 Antibody (aa323-372); Rabbit Polyclonal (IgG) to Human SMAD6; SMAD6; AOVD2; HSMAD6; HsT17432; SMAD 6; MADH7; Mothers against DPP homolog 6; SMAD family member 6; MAD homolog 6; MADH6; anti-SMAD6 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Smad6 antibody detects endogenous levels of Smad6.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
• Sequence Length: 496

• Applicable Applications for anti-SMAD6 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Immunogen: SMAD6 antibody was raised against synthetic peptide derived from human Smad6. (AA Range: 323-372)
• Immunogen Species: Human
• Epitope: aa323-372
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20°C.

WB (Western Blot)

(Western blot analysis of lysate from mouse brain, using Smad6 antibody.)

IHC (Immunohistochemistry)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-SMAD6 antibody

Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions.

NCBI and Uniprot Product Information

• NCBI GI #: 92859871
• NCBI GeneID: 4091
• NCBI Accession #: NP_005576.3
• NCBI GenBank Nucleotide #: NM_005585.4
• UniProt Accession #: O43541; O43654; Q15799; Q7Z7L4; Q96E31; Q9UKZ3; A9J6M5
• Molecular Weight: Predicted MW: 53 kDA
• NCBI Official Full Name: mothers against decapentaplegic homolog 6
• NCBI Official Synonym Full Names: SMAD family member 6
• NCBI Official Symbol: SMAD6
• NCBI Official Synonym Symbols: AOVD2; MADH6; MADH7; HsT17432
• NCBI Protein Information: mothers against decapentaplegic homolog 6
• UniProt Protein Name: Mothers against decapentaplegic homolog 6
• UniProt Gene Name: SMAD6
• UniProt Synonym Gene Names: MADH6; MAD homolog 6; Mothers against DPP homolog 6; SMAD 6; Smad6; hSMAD6