IP (Immunoprecipitation)
(Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody AAA211310 (lot AAA211310-2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211310-1). For blotting immunoprecipitated SMARCA1, AAA211310 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human SMARCA1/SNF2L Polyclonal Antibody | anti-SMARCA1/SNF2L antibody
Rabbit anti-SMARCA1/SNF2L Antibody, Affinity Purified
Gene Names
SMARCA1; SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; NURF140
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
SMARCA1/SNF2L, Antibody; Rabbit anti-SMARCA1/SNF2L Antibody, Affinity Purified; SNF2LB; SWI2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1; SWI; sucrose nonfermenting 2-like protein 1; SNF2-like 1; SNF2L1; probable global transcription activator SNF2L1; NURF140; nucleosome-remodeling factor subunit SNF2L; ISWI; hSNF2L; global transcription activator homologous sequence; ATP-dependent helicase SMARCA1; SNF2L; SNF2LT; Probable global transcription activator SNF2L1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1; anti-SMARCA1/SNF2L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SMARCA1/SNF2L antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse
Immunogen
Between 1004 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody AAA211310 (lot AAA211310-2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211310-1). For blotting immunoprecipitated SMARCA1, AAA211310 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SMARCA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA1 antibody AAA211310 (lot AAA211310-2) used for IP at 6 ug per reaction. SMARCA1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211310-1). For blotting immunoprecipitated SMARCA1, AAA211310 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemistry)
(Detection of human SMARCA1/SNF2L by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMARCA1/SNF2L (Cat. No. AAA211310 Lot2) used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human SMARCA1/SNF2L by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SMARCA1/SNF2L (Cat. No. AAA211310 Lot2) used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB)
Related Product Information for anti-SMARCA1/SNF2L antibody
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. Member of this family have helicase and ATPase activities that alter chromatin structure to facilitate gene transcription. SMARCA1/SNF2L is a component of the human NURF (nucleosome remodeling factor) involved in the regulation of genes involved in neuronal development. SMARCA1/SNF2L is also a component of the heterodimeric complex CERF [CECR2(cat eye syndrome chromosome region, candidate 2)-containing remodeling factor].
Product Categories/Family for anti-SMARCA1/SNF2L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,605 Da
NCBI Official Full Name
probable global transcription activator SNF2L1 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
NCBI Official Symbol
SMARCA1
NCBI Official Synonym Symbols
SWI; ISWI; SWI2; SNF2L; SNF2L1; SNF2LB; SNF2LT; NURF140
NCBI Protein Information
probable global transcription activator SNF2L1; SNF2-like 1; ATP-dependent helicase SMARCA1; sucrose nonfermenting 2-like protein 1; nucleosome-remodeling factor subunit SNF2L; global transcription activator homologous sequence; SWI/SNF-related matrix-ass
UniProt Protein Name
Probable global transcription activator SNF2L1
UniProt Gene Name
SMARCA1
UniProt Synonym Gene Names
SNF2L; SNF2L1
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Product Notes
The SMARCA1/SNF2L smarca1 (Catalog #AAA211310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SMARCA1/SNF2L Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCA1/SNF2L can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the SMARCA1/SNF2L smarca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCA1/SNF2L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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