IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Rabbit anti-Human SMARCC2 Polyclonal Antibody | anti-SMARCC2 antibody
Rabbit anti-Human SMARCC2 Polyclonal Antibody
Gene Names
SMARCC2; Rsc8; BAF170; CRACC2
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
SMARCC2, Antibody; Rabbit anti-Human SMARCC2 Polyclonal Antibody; SWI/SNF complex subunit SMARCC2 (BRG1-associated factor 170) (BAF170) (SWI/SNF complex 170kDa subunit) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2); SMARCC2; BAF170; SMARCC2 Antibody; anti-SMARCC2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid. Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-SMARCC2 antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human SWI/SNF complex subunit SMARCC2 protein (300-650aa)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in: HL60 whole cell lysateAll lanes: SMARCC2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133, 125, 127 kDaObserved band size: 170 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: HL60 whole cell lysateAll lanes: SMARCC2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 133, 125, 127 kDaObserved band size: 170 kDa)
Related Product Information for anti-SMARCC2 antibody
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Product Categories/Family for anti-SMARCC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133kDa
NCBI Official Full Name
SWI/SNF complex subunit SMARCC2 isoform c
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
NCBI Official Symbol
SMARCC2
NCBI Official Synonym Symbols
Rsc8; BAF170; CRACC2
NCBI Protein Information
SWI/SNF complex subunit SMARCC2; SWI3-like protein; BRG1-associated factor 170; SWI/SNF complex 170 kDa subunit; chromatin remodeling complex BAF170 subunit; mammalian chromatin remodeling complex BRG1-associated factor 170; SWI/SNF-related matrix-associa
UniProt Protein Name
SWI/SNF complex subunit SMARCC2
UniProt Gene Name
SMARCC2
UniProt Synonym Gene Names
BAF170; BAF170
UniProt Entry Name
SMRC2_HUMAN
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Product Notes
The SMARCC2 smarcc2 (Catalog #AAA244119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human SMARCC2 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCC2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SMARCC2 smarcc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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