WB (Western Blot)
(SMARCC2 / BAF170 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SMARCC2 / BAF170 antibody (1:500 dilution).)
Rabbit anti-Human, Mouse SMARCC2/BAF170 Polyclonal Antibody | anti-SMARCC2/BAF170 antibody
SMARCC2/BAF170 antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Affinity Purified
Synonyms
SMARCC2/BAF170, Antibody; SMARCC2/BAF170 antibody (pAb); anti-SMARCC2/BAF170 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SMARCC2/BAF170 antibody
WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry)
Immunogen
This antibody was raised against a peptide within the C-terminal region of mouse SMARCC2/BAF170.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(SMARCC2 / BAF170 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SMARCC2 / BAF170 antibody (1:500 dilution).)
WB (Western Blot)
(SMARCC2 / BAF170 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SMARCC2 / BAF170 antibody (1:500 dilution).)
IF (Immunofluorescence)
(SMARCC2 / BAF170 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCC2 / BAF170 antibody at a 1:2,000 dilution.)
IF (Immunofluorescence)
(SMARCC2 / BAF170 antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with SMARCC2 / BAF170 antibody at a 1:2,000 dilution.)
Related Product Information for anti-SMARCC2/BAF170 antibody
Short Description: Our SMARCC2/BAF170 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot; it has been shown to react with Human and Mouse samples.
Background: SMARCC2/BAF170 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 2) is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Background: SMARCC2/BAF170 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 2) is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Product Categories/Family for anti-SMARCC2/BAF170 antibody
NCBI and Uniprot Product Information
Molecular Weight
170kDa
Similar Products
Product Notes
The SMARCC2/BAF170 (Catalog #AAA60029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMARCC2/BAF170 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMARCC2/BAF170 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the SMARCC2/BAF170 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMARCC2/BAF170, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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