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ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-SMARCC2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.)
Rabbit anti-Human, Mouse SWI/SNF complex subunit SMARCC2 Polyclonal Antibody | anti-SMARCC2 antibody
Rabbit anti-human SWI/SNF complex subunit SMARCC2 polyclonal Antibody(SMARCC2)
Gene Names
SMARCC2; Rsc8; BAF170; CRACC2
Reactivity
Human, Mouse
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
SWI/SNF complex subunit SMARCC2, Antibody; Rabbit anti-human SWI/SNF complex subunit SMARCC2 polyclonal Antibody(SMARCC2); BRG1-associated factor 170; BAF170; SWI/SNF complex 170 kDa subunit; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2; SMARCC2; anti-SMARCC2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Sequence Length
1152
Applicable Applications for anti-SMARCC2 antibody
ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate
Non-conjugated
Immunogen
Recombinant human SWI/SNF complex subunit SMARCC2 protein(300-650aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9742 / sc-9744 / sc-10757
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-SMARCC2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.)
ChIP (Chromatin immunoprecipitation)
(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-SMARCC2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA119409 at dilution 1:100)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsil using AAA119409 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human epityphlon using AAA119409 at dilution 1:100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human epityphlon using AAA119409 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human rectal cancer using AAA119409 at dilution 1:100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human rectal cancer using AAA119409 at dilution 1:100)
WB (Western Blot)
(All lanes: SWI/SNF complex subunit SMARCC2 antibody at 2ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 133,125,127 kDaObserved band size: 133 kDa)
WB (Western Blot)
(All lanes: SWI/SNF complex subunit SMARCC2 antibody at 2ug/ml+mouse liver tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 133,125,127 kDaObserved band size: 133 kDa)
Related Product Information for anti-SMARCC2 antibody
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
126,924 Da
NCBI Official Full Name
SWI/SNF complex subunit SMARCC2 isoform c
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
NCBI Official Symbol
SMARCC2
NCBI Official Synonym Symbols
Rsc8; BAF170; CRACC2
NCBI Protein Information
SWI/SNF complex subunit SMARCC2
UniProt Protein Name
SWI/SNF complex subunit SMARCC2
UniProt Gene Name
SMARCC2
UniProt Synonym Gene Names
BAF170; BAF170
UniProt Entry Name
SMRC2_HUMAN
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Product Notes
The SMARCC2 smarcc2 (Catalog #AAA119409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human SWI/SNF complex subunit SMARCC2 polyclonal Antibody(SMARCC2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SWI/SNF complex subunit SMARCC2 can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SMARCC2 smarcc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SWI/SNF complex subunit SMARCC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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