Loading...

Skip to main content
Call us at +1-800-604-9114 for more information about our products or contact us online
product-image-AAA10972_IHC7.jpg IHC (Immunohistochemistry) (Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml)

Rabbit SMURF2 Polyclonal Antibody | anti-SMURF2 antibody

SMURF2 Antibody

Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
Purity
SMURF2 antibody is affinity chromatography purified via peptide column.
Synonyms
SMURF2, Antibody; SMURF2 Antibody; SMURF2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; hSMURF2; SMAD specific E3 ubiquitin protein ligase 2; anti-SMURF2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
Purity/Purification
SMURF2 antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
748
Applicable Applications for anti-SMURF2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF)

SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml.Antibody can also be used for Immunohistochemistry at 2 ug/mL.
Application Notes
SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in humanand mouse samples and Immunofluorescence in human and mouse samples. All otherapplications and species not yet tested.
Conjugate
Unconjugated
Immunogen
SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
Buffer
SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml)

product-image-AAA10972_IHC7.jpg IHC (Immunohistochemistry) (Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/ml. Green: SMURF2 Antibody (AAA10972) Blue: DAPI staining.)

product-image-AAA10972_IF6.jpg IF (Immunofluorescence) (Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/ml. Green: SMURF2 Antibody (AAA10972) Blue: DAPI staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 μg/ml.)

product-image-AAA10972_IHC5.gif IHC (Immunohistochemistry) (Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 μg/ml.Green: SMURF2 Antibody (AAA10972)Blue: DAPI staining)

product-image-AAA10972_IF4.gif IF (Immunofluorescence) (Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 μg/ml.Green: SMURF2 Antibody (AAA10972)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/mL.)

product-image-AAA10972_IF3.jpg IF (Immunofluorescence) (Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 μg/mL.)

product-image-AAA10972_IHC2.jpg IHC (Immunohistochemistry) (Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 μg/mL.)

WB (Western Blot)

(SMURF2 Antibody)

product-image-AAA10972_WB.gif WB (Western Blot) (SMURF2 Antibody)
Related Product Information for anti-SMURF2 antibody
SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 82 kDa

Observed: 85 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase SMURF2
NCBI Official Synonym Full Names
SMAD specific E3 ubiquitin protein ligase 2
NCBI Official Symbol
SMURF2
NCBI Protein Information
E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
UniProt Protein Name
E3 ubiquitin-protein ligase SMURF2
UniProt Gene Name
SMURF2
UniProt Synonym Gene Names
hSMURF2
UniProt Entry Name
SMUF2_HUMAN

Similar Products

Product Notes

The SMURF2 smurf2 (Catalog #AAA10972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMURF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SMURF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF) SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml.Antibody can also be used for Immunohistochemistry at 2 ug/mL. SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in humanand mouse samples and Immunofluorescence in human and mouse samples. All otherapplications and species not yet tested. Researchers should empirically determine the suitability of the SMURF2 smurf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMURF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.