Rabbit SMURF2 Polyclonal Antibody | anti-SMURF2 antibody

SMURF2 Antibody

Cat. Num. AAA10972

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunohistochemistry
• Purity: SMURF2 antibody is affinity chromatography purified via peptide column.
• Synonyms: SMURF2, Antibody; SMURF2 Antibody; SMURF2; E3 ubiquitin-protein ligase SMURF2; SMAD ubiquitination regulatory factor 2; hSMURF2; SMAD specific E3 ubiquitin protein ligase 2; anti-SMURF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.
• Purity/Purification: SMURF2 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 748

• Applicable Applications for anti-SMURF2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC-P), Immunofluorescence (IF); ; SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml.Antibody can also be used for Immunohistochemistry at 2 ug/mL.
• Application Notes: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL.; ; Antibody validated: Western Blot in mouse samples; Immunohistochemistry in humanand mouse samples and Immunofluorescence in human and mouse samples. All otherapplications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.
• Buffer: SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 ug/ml)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 ug/ml. Green: SMURF2 Antibody (AAA10972) Blue: DAPI staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 μg/ml.Green: SMURF2 Antibody (AAA10972)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 μg/mL.)

WB (Western Blot)

(SMURF2 Antibody)

Related Product Information for anti-SMURF2 antibody

SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).

NCBI and Uniprot Product Information

• NCBI GI #: 12232397
• NCBI GeneID: 64750
• NCBI Accession #: NP_073576
• NCBI GenBank Nucleotide #: NM_022739.3
• UniProt Accession #: Q9HAU4; Q52LL1; Q9H260
• Molecular Weight: Predicted: 82 kDa; ; Observed: 85 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase SMURF2
• NCBI Official Synonym Full Names: SMAD specific E3 ubiquitin protein ligase 2
• NCBI Official Symbol: SMURF2
• NCBI Protein Information: E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2
• UniProt Protein Name: E3 ubiquitin-protein ligase SMURF2
• UniProt Gene Name: SMURF2
• UniProt Synonym Gene Names: hSMURF2
• UniProt Entry Name: SMUF2_HUMAN