FCM/FACS (Flow Cytometry)
(STUB1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit STUB1 Polyclonal Antibody | anti-STUB1 antibody
STUB1 Antibody (C-term)
Gene Names
STUB1; CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STUB1, Antibody; STUB1 Antibody (C-term); E3 ubiquitin-protein ligase CHIP; 632-; Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1 {ECO:0000312|HGNC:HGNC:11427}; STUB1 HGNC:11427); anti-STUB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-263 amino acids from the C-terminal region of human STUB1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
236-263
Sequence Length
231
Applicable Applications for anti-STUB1 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(STUB1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(STUB1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(STUB1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the STUB1 antibody detected the STUB1 protein (arrow).)
WB (Western Blot)
(STUB1 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the STUB1 antibody detected the STUB1 protein (arrow).)
Related Product Information for anti-STUB1 antibody
STUB1, or CHIP, is a ubiquitin ligase/cochaperone that
participates in protein quality control by targeting a broad range
of chaperone protein substrates for degradation (Min et al., 2008
[PubMed 18411298]).
participates in protein quality control by targeting a broad range
of chaperone protein substrates for degradation (Min et al., 2008
[PubMed 18411298]).
Product Categories/Family for anti-STUB1 antibody
References
Stankiewicz, M., et al. FEBS J. 277(16):3353-3367(2010)
Naito, A.T., et al. Circ. Res. 106(11):1692-1702(2010)
Loffek, S., et al. Hum. Mutat. 31(4):466-476(2010)
Graf, C., et al. Biochemistry 49(10):2121-2129(2010)
Schulke, J.P., et al. PLoS ONE 5 (7), E11717 (2010) :
Naito, A.T., et al. Circ. Res. 106(11):1692-1702(2010)
Loffek, S., et al. Hum. Mutat. 31(4):466-476(2010)
Graf, C., et al. Biochemistry 49(10):2121-2129(2010)
Schulke, J.P., et al. PLoS ONE 5 (7), E11717 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34856
NCBI Official Full Name
E3 ubiquitin-protein ligase CHIP isoform a
NCBI Official Synonym Full Names
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
NCBI Official Symbol
STUB1
NCBI Official Synonym Symbols
CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
NCBI Protein Information
E3 ubiquitin-protein ligase CHIP
UniProt Protein Name
E3 ubiquitin-protein ligase CHIP
UniProt Gene Name
STUB1
UniProt Entry Name
CHIP_HUMAN
Similar Products
Product Notes
The STUB1 stub1 (Catalog #AAA289857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 236-263. The STUB1 Antibody (C-term) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's STUB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the STUB1 stub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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