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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit STUB1 Polyclonal Antibody | anti-STUB1 antibody
STUB1 Antibody (C-term)
Gene Names
STUB1; CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STUB1, Antibody; STUB1 Antibody (C-term); E3 ubiquitin-protein ligase CHIP; 632-; Antigen NY-CO-7; CLL-associated antigen KW-8; Carboxy terminus of Hsp70-interacting protein; STIP1 homology and U box-containing protein 1 {ECO:0000312|HGNC:HGNC:11427}; STUB1 HGNC:11427); anti-STUB1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 203-231 amino acids from the C-terminal region of human STUB1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
203-231
Sequence Length
231
Applicable Applications for anti-STUB1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHIP (STUB1) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(CHIP Antibody (C-term) western blot analysis in 293 cell line and human placenta lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).)
WB (Western Blot)
(CHIP Antibody (C-term) western blot analysis in 293 cell line and human placenta lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).)
WB (Western Blot)
(CHIP Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).)
WB (Western Blot)
(CHIP Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHIP antibody detected the CHIP protein (arrow).)
Related Product Information for anti-STUB1 antibody
CHIP is an E3 ligase for nNOS whose action is facilitated by (and possibly requires) its interaction with nNOS-bound hsp70. Co-chaperone CHIP, possibly with another E3 ligase(s), modulates the ubiquitylation of mutant Cu/Zn-superoxide dismutase and renders them more susceptible for proteasomal degradation. CHIP functions as a negative regulator of AR transcriptional activity by promoting AR degradation. CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival. CHIP can interact with the Smad1/Smad4 proteins and block BMP signal transduction through the ubiquitin-mediated degradation of Smad proteins. CHIP E3 controls both the association of Hsp70/Hsp90 chaperones with ErbB2 and the down-regulation of ErbB2 induced by inhibitors of Hsp90. CHIP is associated with Parkin and enhances its ubiquitin ligase activity related to Parkinson's disease.
Product Categories/Family for anti-STUB1 antibody
References
Peng,H.M., et al. J. Biol. Chem. 279 (51), 52970-52977 (2004)
Alberti,S., et al. Mol. Biol. Cell 15 (9), 4003-4010 (2004)
Beausoleil,S.A., et al. PNAS 101 (33), 12130-12135 (2004)
He,B., et al. J. Biol. Chem. 279 (29), 30643-30653 (2004)
Petrucelli,L., et al. Hum. Mol. Genet. 13 (7), 703-714 (2004)
Shimura,H., et al. J. Biol. Chem. 279 (6), 4869-4876 (2004)
Li,L., et al. Mol. Cell. Biol. 24 (2), 856-864 (2004)
Zhou,P., et al. J. Biol. Chem. 278 (16), 13829-13837 (2003)
Imai,Y., et al. Mol. Cell 10 (1), 55-67 (2002)
Ballinger,C.A., et al. Mol. Cell. Biol. 19 (6), 4535-4545 (1999)
Alberti,S., et al. Mol. Biol. Cell 15 (9), 4003-4010 (2004)
Beausoleil,S.A., et al. PNAS 101 (33), 12130-12135 (2004)
He,B., et al. J. Biol. Chem. 279 (29), 30643-30653 (2004)
Petrucelli,L., et al. Hum. Mol. Genet. 13 (7), 703-714 (2004)
Shimura,H., et al. J. Biol. Chem. 279 (6), 4869-4876 (2004)
Li,L., et al. Mol. Cell. Biol. 24 (2), 856-864 (2004)
Zhou,P., et al. J. Biol. Chem. 278 (16), 13829-13837 (2003)
Imai,Y., et al. Mol. Cell 10 (1), 55-67 (2002)
Ballinger,C.A., et al. Mol. Cell. Biol. 19 (6), 4535-4545 (1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34856
NCBI Official Full Name
E3 ubiquitin-protein ligase CHIP isoform a
NCBI Official Synonym Full Names
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
NCBI Official Symbol
STUB1
NCBI Official Synonym Symbols
CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
NCBI Protein Information
E3 ubiquitin-protein ligase CHIP
UniProt Protein Name
E3 ubiquitin-protein ligase CHIP
UniProt Gene Name
STUB1
UniProt Entry Name
CHIP_HUMAN
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Product Notes
The STUB1 stub1 (Catalog #AAA284480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 203-231. The STUB1 Antibody (C-term) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's STUB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the STUB1 stub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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