Rabbit anti-Human, Mouse STUB1/CHIP Polyclonal Antibody | anti-STUB1/CHIP antibody

Rabbit anti-STUB1/CHIP Antibody, Affinity Purified

Cat. Num. AAA211539

• Gene Names: STUB1; CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: STUB1/CHIP, Antibody; Rabbit anti-STUB1/CHIP Antibody, Affinity Purified; NY-CO-7; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STIP1 homology and U box-containing protein 1; serologically defined colon cancer antigen 7; SDCCAG7; UBOX1; RING-type E3 ubiquitin transferase CHIP; heat shock protein A binding protein 2 (c-terminal); E3 ubiquitin-protein ligase CHIP; CLL-associated antigen KW-8; CHIP; carboxy terminus of Hsp70-interacting protein; antigen NY-CO-7; HSPABP2; SCAR16; STIP1 homology and U-box containing protein 1; anti-STUB1/CHIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-STUB1/CHIP antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 1 and 50
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse STUB1/CHIP by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-STUB1/CHIP antibody AAA211539 (lot AAA211539-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human STUB1/CHIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-STUB1/CHIP antibody AAA211539 (lot AAA211539-1) used for IP at 3 ug/mg lysate. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody A301-573A, which recognizes a downstream epitope. For blotting immunoprecipitated STUB1/CHIP, AAA211539 was used at 0.1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

Related Product Information for anti-STUB1/CHIP antibody

STUB1/CHIP is an E3 ubiquitin ligase and co-chaperone of heat-shock protein 70 (Hsp70). STUB1/CHIP interacts with Hsp70 to monitor protein folding and direct misfolded proteins for proteasomal degradation. It has been found to also associate with Hsp90 to remove phosphorylated protein tau, a protein that accumulates in the brains of Alzheimers and Down's syndrome patients. STUB1/CHIP has been reported to also have intrinsic chaperone activity and recognize non-native proteins in response to heat stress. In this way, STUB1/CHIP has been shown to be a direct chaperone for p53.

Product Categories/Family for anti-STUB1/CHIP antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 56181387
• NCBI GeneID: 10273
• NCBI Accession #: NP_005852.2
• NCBI GenBank Nucleotide #: NM_005861.3
• UniProt Accession #: Q9UNE7; O60526; Q969U2; Q9HBT1; A2IDB9
• Molecular Weight: 27,067 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase CHIP isoform a
• NCBI Official Synonym Full Names: STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: STUB1
• NCBI Official Synonym Symbols: CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
• NCBI Protein Information: E3 ubiquitin-protein ligase CHIP; antigen NY-CO-7; CLL-associated antigen KW-8; serologically defined colon cancer antigen 7; carboxy terminus of Hsp70-interacting protein; heat shock protein A binding protein 2 (c-terminal)
• UniProt Protein Name: E3 ubiquitin-protein ligase CHIP
• UniProt Gene Name: STUB1
• UniProt Entry Name: CHIP_HUMAN