Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase CHIP (STUB1) Standard Curve.)
Human E3 Ubiquitin-Protein Ligase CHIP (STUB1) ELISA Kit | STUB1 elisa kit
Human E3 Ubiquitin-Protein Ligase CHIP (STUB1) ELISA Kit
Gene Names
STUB1; CHIP; SCA48; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
Reactivity
Human
Synonyms
E3 Ubiquitin-Protein Ligase CHIP (STUB1); N/A; Human E3 Ubiquitin-Protein Ligase CHIP (STUB1) ELISA Kit; STUB1; LA16c-313D11.6; CHIP; HSPABP2; NY-CO-7; SDCCAG7; UBOX1; heat shock protein A binding protein 2 (c-terminal); serologically defined colon cancer antigen 7; STUB1 elisa kit
Reactivity
Human
Specificity
Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human STUB1. No significant cross-reactivity or interference between Human STUB1 and analogues was observed.
Sequence Length
303
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase CHIP (STUB1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase CHIP (STUB1) Standard Curve.)
Related Product Information for STUB1 elisa kit
Description: This Human E3 ubiquitin-protein ligase CHIP (STUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate STUB1 in samples. An antibody specific for STUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The CHIP protein encoded by this gene binds to and inhibits the ATPase activity of the chaperone proteins HSC70 and HSP70 and blocks the forward reaction of the HSC70-HSP70 substrate-binding cycle. In addition, CHIP possesses E3 ubiquitin ligase activity and promotes ubiquitylation. CHIP enhances HSP70 induction during acute stress and also mediates its turnover during the stress recovery process. The deduced 303-amino acid proteinhas a molecular mass of 35 kD and contains three 34-amino acid TPR domains at its N terminus, a central domain rich in charged residues, and 2 potential nuclear localization signals. Human CHIP shares 97% and 53% amino acid identity with its mouse and Drosophila homologs, respectively, with the highest conservation in the 94 residues of the C terminus.
Background: The CHIP protein encoded by this gene binds to and inhibits the ATPase activity of the chaperone proteins HSC70 and HSP70 and blocks the forward reaction of the HSC70-HSP70 substrate-binding cycle. In addition, CHIP possesses E3 ubiquitin ligase activity and promotes ubiquitylation. CHIP enhances HSP70 induction during acute stress and also mediates its turnover during the stress recovery process. The deduced 303-amino acid proteinhas a molecular mass of 35 kD and contains three 34-amino acid TPR domains at its N terminus, a central domain rich in charged residues, and 2 potential nuclear localization signals. Human CHIP shares 97% and 53% amino acid identity with its mouse and Drosophila homologs, respectively, with the highest conservation in the 94 residues of the C terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase CHIP
NCBI Official Synonym Full Names
STIP1 homology and U-box containing protein 1
NCBI Official Symbol
STUB1
NCBI Official Synonym Symbols
CHIP; SCA48; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
NCBI Protein Information
E3 ubiquitin-protein ligase CHIP
UniProt Protein Name
E3 ubiquitin-protein ligase CHIP
UniProt Gene Name
STUB1
UniProt Entry Name
CHIP_HUMAN
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Product Notes
The Human STUB1 stub1 (Catalog #AAA330613) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330613 ELISA Kit recognizes Human STUB1. It is sometimes possible for the material contained within the vial of "E3 Ubiquitin-Protein Ligase CHIP (STUB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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