WB (Western Blot)
(Western blot analysis Synphilin-1 using LOVO whole cell lysates)
Rabbit anti-Human, Mouse Synphilin-1 Polyclonal Antibody | anti-SNCAIP antibody
Synphilin-1 Antibody
Gene Names
SNCAIP; Sph1; SYPH1
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Synphilin-1, Antibody; Synphilin-1 Antibody; Alpha synuclein interacting protein; Alpha-synuclein-interacting protein; MGC39814; SNCAIP; SNCAP_HUMAN; Sph1; Synphilin-1; Synphilin1; Synuclein alpha interacting protein (synphilin); Synuclein alpha interacting protein; SYPH 1; anti-SNCAIP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Synphilin-1 antibody detects endogenous levels of total Synphilin-1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
603
Applicable Applications for anti-SNCAIP antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Synphilin-1
Subcellular Location
Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA.
Tissue Specificity
Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis Synphilin-1 using LOVO whole cell lysates)
WB (Western Blot)
(Western blot analysis Synphilin-1 using LOVO whole cell lysates)
IHC (Immunohistochemistry)
(AAA327553 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327553 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SNCAIP antibody
Function: Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1.
Subunit Structure: Homodimer (Probable). Heterodimer of isoform 1 and isoform 2 (Probable). Interacts with SIAH1, SIAH2, SNCA, RNF19A AND PRKN. Isoform 2 has a strong tendency to form aggregates and can sequester isoform 1.
Post-translational Modifications: Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
Subunit Structure: Homodimer (Probable). Heterodimer of isoform 1 and isoform 2 (Probable). Interacts with SIAH1, SIAH2, SNCA, RNF19A AND PRKN. Isoform 2 has a strong tendency to form aggregates and can sequester isoform 1.
Post-translational Modifications: Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 100 kDa
Predicted: 101 kDa
Predicted: 101 kDa
NCBI Official Full Name
synphilin-1 isoform 1A
NCBI Official Synonym Full Names
synuclein alpha interacting protein
NCBI Official Symbol
SNCAIP
NCBI Official Synonym Symbols
Sph1; SYPH1
NCBI Protein Information
synphilin-1
UniProt Protein Name
Synphilin-1
UniProt Gene Name
SNCAIP
UniProt Synonym Gene Names
Sph1
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Product Notes
The SNCAIP sncaip (Catalog #AAA327553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Synphilin-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Synphilin-1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SNCAIP sncaip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synphilin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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