Rabbit anti-Human, mouse TAF1D Polyclonal Antibody | anti-TAF1D antibody

TAF1D Antibody (N-term)

Cat. Num. AAA284834

• Gene Names: TAF1D; JOSD3; RAFI41; TAFI41; TAF(I)41
• Reactivity: Human, mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TAF1D, Antibody; TAF1D Antibody (N-term); TATA box-binding protein-associated factor RNA polymerase I subunit D; RNA polymerase I-specific TBP-associated factor 41 kDa; TAFI41; TATA box-binding protein-associated factor 1D; TBP-associated factor 1D; Transcription initiation factor SL1/TIF-IB subunit D; TAF1D; JOSD3; anti-TAF1D antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB27052
• Specificity: This TAF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human TAF1D.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Sequence Positions: 62-90
• Sequence Length: 278

• Applicable Applications for anti-TAF1D antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Function: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits.
• Cellular Location: Nucleus EMBL; AK027016; BAB15628.1; -; mRNA EMBL; CR457369; CAG33650.1; -; mRNA EMBL; BC001972; AAH01972.1; -; mRNA CCDS; CCDS8293.1; - RefSeq; NP_077021.1; NM_024116.3 UniGene; Hs.355750; - UniGene; Hs.732267; - ProteinModelPortal; Q9H5J8; - SMR; Q9H5J8; - BioGrid; 122548; 56 IntAct; Q9H5J8; 42 MINT; Q9H5J8; - STRING; 9606.ENSP00000314971; - iPTMnet; Q9H5J8; - PhosphoSitePlus; Q9H5J8; - BioMuta; TAF1D; - DMDM; 74733586; - EPD; Q9H5J8; - jPOST; Q9H5J8; - MaxQB; Q9H5J8; - PaxDb; Q9H5J8; - PeptideAtlas; Q9H5J8; - PRIDE; Q9H5J8; - ProteomicsDB; 80916; - DNASU; 79101; - Ensembl; ENST00000323981; ENSP00000314971; ENSG00000166012 Ensembl; ENST00000448108; ENSP00000410409; ENSG00000166012 Ensembl; ENST00000526015; ENSP00000435087; ENSG00000166012 GeneID; 79101; - KEGG; hsa:79101; - UCSC; uc001ped.5; human CTD; 79101; - DisGeNET; 79101; - EuPathDB; HostDB:ENSG00000166012.14; - GeneCards; TAF1D; - HGNC; HGNC:28759; TAF1D HPA; HPA055935; - HPA; HPA060385; - MIM; 612823; gene neXtProt; NX_Q9H5J8; - OpenTargets; ENSG00000166012; - PharmGKB; PA164726421; - eggNOG; ENOG410IZI3; Eukaryota eggNOG; ENOG41124VA; LUCA GeneTree; ENSGT00390000009061; - HOGENOM; HOG000154498; - HOVERGEN; HBG096071; - InParanoid; Q9H5J8; - KO; K15215; - OMA; NEDFDSR; - OrthoDB; 1263117at2759; - PhylomeDB; Q9H5J8; - TreeFam; TF335756; - Reactome; R-HSA-427359; SIRT1 negatively regulates rRNA expression Reactome; R-HSA-427413; NoRC negatively regulates rRNA expression Reactome; R-HSA-5250924; B-WICH complex positively regulates rRNA expression Reactome; R-HSA-73762; RNA Polymerase I Transcription Initiation Reactome; R-HSA-73772; RNA Polymerase I Promoter Escape Reactome; R-HSA-73777; RNA Polymerase I Chain Elongation Reactome; R-HSA-73863; RNA Polymerase I Transcription Termination ChiTaRS; TAF1D; human GenomeRNAi; 79101; - PRO; PR:Q9H5J8; - Proteomes; UP000005640; Chromosome 11 Bgee; ENSG00000166012; Expressed in 227 organ(s), highest expression level in visceral pleura ExpressionAtlas; Q9H5J8; baseline and differential Genevisible; Q9H5J8; HS GO; GO:0005815; C:microtubule organizing center; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005668; C:RNA polymerase transcription factor SL1 complex; IEA:InterPro GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW GO; GO:0045815; P:positive regulation of gene expression, epigenetic; TAS:Reactome GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro GO; GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome GO; GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome InterPro; IPR027976; TAF1D PANTHER; PTHR14562; PTHR14562; 1 Pfam; PF15333; TAF1D; 1 1: Evidence at protein level; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation CHAIN 1 278 TATA box-binding protein-associated factor RNA polymerase I subunit D /FTId=PRO_0000250717 MOD_RES 23 23 Phosphoserine MOD_RES 138 138 Phosphoserine MOD_RES 234 234 Phosphoserine CONFLICT 14 14 S -> T (in Ref. 3; CAG33650) CONFLICT 97 97 R -> G (in Ref. 3; CAG33650) CONFLICT 278 278 M -> I (in Ref. 3; CAG33650) SEQUENCE 278 AA; 32058 MW; 4BA37C73BB387446 CRC64; MDKSGIDSLD HVTSDAVELA NRSDNSSDSS LFKTQCIPYS PKGEKRNPIR KFVRTPESVH ASDSSSDSSF EPIPLTIKAI FERFKNRKKR YKKKKKRRYQ PTGRPRGRPE GRRNPIYSLI DKKKQFRSRG SGFPFLESEN EKNAPWRKIL TFEQAVARGF FNYIEKLKYE HHLKESLKQM NVGEDLENED FDSRRYKFLD DDGSISPIEE STAEDEDATH LEDNECDIKL AGDSFIVSSE FPVRLSVYLE EEDITEEAAL SKKRATKAKN TGQRGLKM
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(TAF1D Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(TAF1D Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TAF1D Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(TAF1D Antibody (N-term) western blot analysis in NCI-H292,Jurkat cell line and mouse liver lysates (15ug/lane).This demonstrates the TAF1D antibody detected the TAF1D protein (arrow).)

Related Product Information for anti-TAF1D antibody

TAF1D is a member of the SL1 complex, which includes TBP (MIM 600075) and TAF1A (MIM 604903), TAF1B (MIM 604904), and TAF1C (MIM 604905), and plays a role in RNA polymerase I transcription (Wang et al., 2004 [PubMed 15520167]; Gorski et al., 2007 [PubMed 17318177]).

References

Gorski, J.J., et al. EMBO J. 26(6):1560-1568(2007)
Wang, L., et al. Cancer Res. 64(21):7673-7677(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 13129136
• NCBI GeneID: 79101
• NCBI Accession #: NP_077021.1
• NCBI GenBank Nucleotide #: NM_024116.3
• UniProt Accession #: Q9H5J8; Q6I9Y6
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit D
• NCBI Official Synonym Full Names: TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa
• NCBI Official Symbol: TAF1D
• NCBI Official Synonym Symbols: JOSD3; RAFI41; TAFI41; TAF(I)41
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit D
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit D
• UniProt Gene Name: TAF1D
• UniProt Synonym Gene Names: JOSD3; TAFI41; TBP-associated factor 1D
• UniProt Entry Name: TAF1D_HUMAN