WB (Western Blot)
(Detection of human TCP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody AAA212168 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
Rabbit anti-Human TCP1 Polyclonal Antibody | anti-TCP1 antibody
Rabbit anti-TCP1 Antibody, Affinity Purified
Gene Names
TCP1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TCP1, Antibody; Rabbit anti-TCP1 Antibody, Affinity Purified; t-complex 1 protein; tailless complex polypeptide 1; TCP-1-alpha; T-complex protein 1, alpha subunit; D6S230E; CCT1; CCT-alpha; CCTa; T-complex protein 1 subunit alpha; t-complex 1; anti-TCP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-TCP1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 506 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TCP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody AAA212168 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
WB (Western Blot)
(Detection of human TCP1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TCP1 antibody AAA212168 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. TCP1 was also immunoprecipitated by rabbit anti-TCP1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).)
IHC (Immunohiostchemistry)
(Detection of human TCP1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. AAA212168 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human TCP1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. AAA212168 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TCP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. Lot1, left panel and AAA212168 Lot1, right panel) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TCP1 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCP1 (Cat. No. Lot1, left panel and AAA212168 Lot1, right panel) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-TCP1 antibody
T-complex protein 1 subunit alpha (TCP1-alpha) is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin [taken from NCBI Entrez Gene (Gene ID: 6950)].
Product Categories/Family for anti-TCP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,344 Da
NCBI Official Full Name
T-complex protein 1 subunit alpha isoform a
NCBI Official Synonym Full Names
t-complex 1
NCBI Official Symbol
TCP1
NCBI Official Synonym Symbols
CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
NCBI Protein Information
T-complex protein 1 subunit alpha; T-complex protein 1, alpha subunit; tailless complex polypeptide 1
UniProt Protein Name
T-complex protein 1 subunit alpha
UniProt Gene Name
TCP1
UniProt Synonym Gene Names
CCT1; CCTA; TCP-1-alpha
UniProt Entry Name
TCPA_HUMAN
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Product Notes
The TCP1 tcp1 (Catalog #AAA212168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TCP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCP1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TCP1 tcp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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