Rabbit anti-Human, Rat TFEB Polyclonal Antibody | anti-TFEB antibody

TFEB Antibody

Cat. Num. AAA26969

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: TFEB, Antibody; TFEB Antibody; Transcription factor EB, Transcription factor EB (Class E basic helix-loop-helix protein 35) (bHLHe35), TFEB, BHLHE35; anti-TFEB antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

• Applicable Applications for anti-TFEB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Recommended dilution; WB: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:50-1:200
• Immunogen: Recombinant Human Transcription factor EB protein (1-476AA)
• Immunogen Species: Homo sapiens (Human)
• Research Area: Tags & Cell Markers
• Target Names: TFEB
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

WB (Western Blot)

(Western BlotPositive WB detected in: Rat lung tissueAll lanes: TFEB antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53, 44 KDaObserved band size: 70 KDa)

IF (Immunofluorescence)

(Immunofluorescent analysis of Hela cells using AAA26969 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human placenta tissue using AAA26969 at dilution of 1:100)

Related Product Information for anti-TFEB antibody

Relevance: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in Tcell-dependent antibody responses in activated CD4(+) T-cells and thymusdependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

NCBI and Uniprot Product Information

• NCBI GI #: 429836865
• NCBI GeneID: 7942
• NCBI Accession #: NP_001161299.2
• NCBI GenBank Nucleotide #: NM_001167827.2
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• NCBI Official Full Name: transcription factor EB isoform 2
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB
• UniProt Protein Name: Transcription factor EB
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN