FCM/FACS (Flow Cytometry)
(TFIIIC90 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Mouse TFIIIC90 Polyclonal Antibody | anti-GTF3C4 antibody
TFIIIC90 Antibody (N-term)
Gene Names
GTF3C4; KAT12; TFIII90; TFIIIC90; TFIIIC290; TFIIICDELTA
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TFIIIC90, Antibody; TFIIIC90 Antibody (N-term); General transcription factor 3C polypeptide 4; TF3C-delta; Transcription factor IIIC 90 kDa subunit; TFIIIC 90 kDa subunit; TFIIIC90; Transcription factor IIIC subunit delta; GTF3C4; anti-GTF3C4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TFIIIC90 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-244 amino acids from the N-terminal region of human TFIIIC90.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.26 (varies by lot)
Sequence Positions
214-244
Sequence Length
822
Applicable Applications for anti-GTF3C4 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TFIIIC90 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TFIIIC90 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(TFIIIC90 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TFIIIC90 antibody detected the TFIIIC90 protein (arrow).)
WB (Western Blot)
(TFIIIC90 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TFIIIC90 antibody detected the TFIIIC90 protein (arrow).)
Related Product Information for anti-GTF3C4 antibody
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
References
Joubert, B.R., et al. Genome Med 2 (3), 17 (2010) :
Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999)
Dumay-Odelot, H., et al. J. Biol. Chem. 282(23):17179-17189(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91982
NCBI Official Full Name
general transcription factor 3C polypeptide 4
NCBI Official Synonym Full Names
general transcription factor IIIC, polypeptide 4, 90kDa
NCBI Official Symbol
GTF3C4
NCBI Official Synonym Symbols
KAT12; TFIII90; TFIIIC90; TFIIIC290; TFIIICDELTA
NCBI Protein Information
general transcription factor 3C polypeptide 4
UniProt Protein Name
General transcription factor 3C polypeptide 4
UniProt Gene Name
GTF3C4
UniProt Synonym Gene Names
TFIIIC 90 kDa subunit; TFIIIC90
UniProt Entry Name
TF3C4_HUMAN
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Product Notes
The GTF3C4 gtf3c4 (Catalog #AAA285664) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 214-244. The TFIIIC90 Antibody (N-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFIIIC90 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the GTF3C4 gtf3c4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFIIIC90, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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