Rabbit TMPRSS2 Polyclonal Antibody | anti-TMPRSS2 antibody

TMPRSS2 (IN) Antibody

Cat. Num. AAA11038

• Gene Names: TMPRSS2; PP9284; PRSS10
• Reactivity: Human, Mouse, Rat; Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).
• Applications: ELISA, Immunofluorescence, Western Blot
• Purity: TMPRSS2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TMPRSS2, Antibody; TMPRSS2 (IN) Antibody; TMPRSS2 Antibody: Transmembrane protease serine 2; Serine protease 10; PRSS10; Transmembrane protease serine 2 non-catalytic chain; Transmembrane protease serine 2 catalytic chain; transmembrane serine protease 2; anti-TMPRSS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TMPRSS2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; TMPRSS2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-TMPRSS2 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: WB: 2ug/mL; IF: 20ug/mL.; ; Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Immunogen: Anti-TMPRSS2 antibody was raised against a peptide corresponding to 13 amino acids near the center of human TMPRSS2.; ; The immunogen is located within amino acids 320-370 of TMPRSS2.
• Conjugation: Unconjugated
• Protein GI#: 115502469
• Isoform: Human TMPRSS2 has 2 isoforms, including isoform 1 (492aa, 54kD) and isoform 2 (529aa, 57.7kD). Mouse TMPRSS2 has 1 isoform (490aa, 54kD). Rat TMPRSS2 has one isoform (490aa, 53kD). 9571 can detect TMPRSS2 in human, mouse and rat.
• NCBI Organism: Homo sapiens
• Preparation and Storage: Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot)

(Figure 3 Western Blot Validation in Rat TissuesLoading: 15 ug of lysates per lane. Antibodies: TMPRSS2, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane.Antibodies: TMPRSS2 9571, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 1 WB Validation in Human Cell Lines Loading: 15 ug of lysate Antibodies: TMPRSS2, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)

Related Product Information for anti-TMPRSS2 antibody

TMPRSS2 Antibody: TMPRSS2 is a plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate. Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells. It facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry. It proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). TMPRSS2 is essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9), and it is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.

Product Categories/Family for anti-TMPRSS2 antibody

Primary Antibody; Infectious Disease; COVID-19

References

Lucas et al. Cancer Discov. 2014; 4(11):1310-25.
Ko et al. Cancer Res. 2015; 75(14):2949-60.
Zang et al. Sci. Immunol. 2020; 5(47):eabc3582.

NCBI and Uniprot Product Information

• NCBI GI #: 227499990
• NCBI GeneID: 7113
• NCBI Accession #: NP_001128571.1
• NCBI GenBank Nucleotide #: NM_001135099.1
• UniProt Accession #: O15393; Q6GTK7; Q9BXX1; A8K6Z8; B2R8E5; B7Z459; D3DSJ2; F8WES1
• Molecular Weight: 57,690 Da
• NCBI Official Full Name: transmembrane protease serine 2 isoform 1
• NCBI Official Synonym Full Names: transmembrane serine protease 2
• NCBI Official Symbol: TMPRSS2
• NCBI Official Synonym Symbols: PP9284; PRSS10
• NCBI Protein Information: transmembrane protease serine 2
• UniProt Protein Name: Transmembrane protease serine 2
• UniProt Gene Name: TMPRSS2
• UniProt Synonym Gene Names: PRSS10