IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP2A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit TOP2A Polyclonal Antibody | anti-TOP2A antibody
TOP2A Antibody (C-term)
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human (Predicted Reactivity: Hamster, Mouse, Rat)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TOP2A, Antibody; TOP2A Antibody (C-term); DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme; TOP2A; TOP2; anti-TOP2A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Hamster, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.415 (varies by lot)
Sequence Positions
1167-1196
Sequence Length
1,612
Applicable Applications for anti-TOP2A antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP2A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of TOP2A Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of TOP2A Antibody (C-term) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of TOP2A Antibody (C-term) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.)
Related Product Information for anti-TOP2A antibody
TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Product Categories/Family for anti-TOP2A antibody
References
Kawamura,R., et.al, J. Cell Biol. 188 (5), 653-663 (2010)
Alchanati,I., et.al, PLoS ONE 4 (12), E8104 (2009)
Alchanati,I., et.al, PLoS ONE 4 (12), E8104 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
174385
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
UniProt Entry Name
TOP2A_HUMAN
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Product Notes
The TOP2A top2a (Catalog #AAA288888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1167-1196. The TOP2A Antibody (C-term) reacts with Human (Predicted Reactivity: Hamster, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TOP2A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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