IHC (Immunohistochemisry)
(TRF2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
Rabbit anti-Human, Rat TRF2 Polyclonal Antibody | anti-TERF2 antibody
Anti-TRF2 Antibody
Gene Names
TERF2; TRF2; TRBF2
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
TRF2, Antibody; Anti-TRF2 Antibody; TERF 2; Terf2; TRBF 2; TRBF2; TRF 2; Q15554; Telomeric repeat-binding factor 2; anti-TERF2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
542
Applicable Applications for anti-TERF2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E Coli-derived human TRF2 recombinant protein (Position: A81-K287). Human TRF2 shares 95.7% amino acid (aa) sequence identity with mouse TRF2.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(TRF2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohistochemisry)
(TRF2 was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(TRF2 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
IHC (Immunohiostchemistry)
(TRF2 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)
WB (Western Blot)
(Western blot analysis of TRF2 expression in rat thymus extract (lane 1) and COLO320 whole cell lysates (lane 2). TRF2 at 75KD was detected using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
WB (Western Blot)
(Western blot analysis of TRF2 expression in rat thymus extract (lane 1) and COLO320 whole cell lysates (lane 2). TRF2 at 75KD was detected using rabbit anti- TRF2 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)
Related Product Information for anti-TERF2 antibody
Rabbit IgG polyclonal antibody for Telomeric repeat-binding factor 2(TERF2) detection.
Background: Telomeric repeat-binding factor 2, also known as TERF2, TRF2, or TRBF2, is encoded in humans by the TERF2 gene. This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.
Background: Telomeric repeat-binding factor 2, also known as TERF2, TRF2, or TRBF2, is encoded in humans by the TERF2 gene. This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic.
References
1. "Entrez Gene: TERF2 telomeric repeat binding factor 2".
2. Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002). "Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases". J. Biol. Chem. 277 (43): 41110-9.
3. Sakaguchi, A. Y.; Padalecki, S. S.; Mattern, V.; Rodriguez, A.; Leach, R. J.; McGill, J. R.; Chavez, M.; Giambernardi, T. A. (1998-05-01). "Chromosomal sublocalization of the transcribed human telomere repeat binding factor 2 gene and comparative mapping in the mouse". Somatic Cell and Molecular Genetics. 24 (3): 157-163.
2. Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002). "Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases". J. Biol. Chem. 277 (43): 41110-9.
3. Sakaguchi, A. Y.; Padalecki, S. S.; Mattern, V.; Rodriguez, A.; Leach, R. J.; McGill, J. R.; Chavez, M.; Giambernardi, T. A. (1998-05-01). "Chromosomal sublocalization of the transcribed human telomere repeat binding factor 2 gene and comparative mapping in the mouse". Somatic Cell and Molecular Genetics. 24 (3): 157-163.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,298 Da
NCBI Official Full Name
telomeric repeat-binding factor 2
NCBI Official Synonym Full Names
telomeric repeat binding factor 2
NCBI Official Symbol
TERF2
NCBI Official Synonym Symbols
TRF2; TRBF2
NCBI Protein Information
telomeric repeat-binding factor 2
UniProt Protein Name
Telomeric repeat-binding factor 2
UniProt Gene Name
TERF2
UniProt Synonym Gene Names
TRBF2; TRF2
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Product Notes
The TERF2 terf2 (Catalog #AAA46722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TRF2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TERF2 terf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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